Anti-SMUG1 antibody (ab15716)
Key features and details
- Goat polyclonal to SMUG1
- Suitable for: Flow Cyt, ICC
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-SMUG1 antibody
See all SMUG1 primary antibodies -
Description
Goat polyclonal to SMUG1 -
Host species
Goat -
Tested applications
Suitable for: Flow Cyt, ICCmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide: PQAFLLGSIHEPA, corresponding to N terminal amino acids 2-14 of SMUG1.
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Positive control
- ICC: U2OS and MCF7 cells; Flow Cyt: MCF7 cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Flow Cytometry - Anti-SMUG1 antibody (ab15716)Flow cytometric analysis of paraformaldehyde fixed MCF7 cells (blue line) labelling SMUG1 with ab15716. Cells permeabilized with 0.5% Triton. Primary incubation 1 hour (10 µg/mL) followed by Alexa Fluor® 488 secondary antibody (1 µg/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor® 488 secondary antibody.
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Immunocytochemistry - Anti-SMUG1 antibody (ab15716)Immunocytochemistry/immunofluorescence analysis of U2OS cells labelling SMUG1 with ab15716 at 10 µg/mL showing strong nuclear staining. Cells were fixed with paraformaldehyde and permeabilized with 0.15% Triton. Primary incubation for 1 hour. Alexa Fluor® 488 secondary antibody at 2 µg/mL (green). Nuclear DNA was labelled with DAPI (blue).
Negative control: Unimmunized goat IgG (10 µg/mL) followed by Alexa Fluor® 488 secondary antibody (2 µg/mL).
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Immunocytochemistry - Anti-SMUG1 antibody (ab15716)Immunocytochemistry/immunofluorescence analysis of MCF7 cells labelling SMUG1 with ab15716 at 10 µg/mL showing strong nuclear and cytoplasmic staining. Cells were fixed with paraformaldehyde and permeabilized with 0.15% Triton. Primary incubation for 1 hour. Alexa Fluor® 488 secondary antibody at 2 µg/mL (green). Nuclear DNA was labelled with DAPI (blue).
Negative control: Unimmunized goat IgG (10 µg/mL) followed by Alexa Fluor® 488 secondary antibody (2 µg/mL).
