Oxalate Oxidase Assay Kit (ab241007)
Key features and details
- Detection method: Fluorescent
- Platform: Microplate reader
Overview
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Product name
Oxalate Oxidase Assay Kit -
Detection method
Fluorescent -
Product overview
Oxalate Oxidase Assay Kit (ab241007) provides a quick and easy method for the measurement of Oxalate Oxidase activity in various samples. In this assay, Oxalate Oxidase converts oxalate into hydrogen peroxide, which in turn, reacts with a probe and converter generating a fluorometric signal (535/587 nm).
The generated fluorescence is directly proportional to the amount of active Oxalate Oxidase present in samples. The assay is simple, sensitive, high–throughput adaptable and can detect less than 4 μU of oxalate Oxidase activity per sample.
The kit allows for the measurement of Oxalate Oxidase activity in plant seeds and plant tissues.
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Platform
Microplate reader
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 100 tests H2O2 Standard (0.88M) 1 x 100µl OxOx Assay Buffer 1 x 25ml OxOx Converter 1 vial OxOx Positive Control 1 vial OxOx Substrate 1 vial Probe (in DMSO) 1 x 200µl -
Relevance
Oxalate Oxidase (OxOx EC 1.2.3.4) belongs to the cupin protein superfamily and catalyzes the conversion of Oxalate into H2O2 and CO2. It plays an important role for stress response in plants. For example, wood rotting fungi generates high levels of oxalate, causing plant rot in many crops including lettuce, soybean, dry bean, tomato, etc. Overexpression of OxOx in plants (e.g. tomato, soybean, lettuce and tobacco) is able to generate transgenic plants showing resistance to fungal pathogenesis. Accurate measurement of Oxalate Oxidase activity is valuable for mechanistic studies in plants and development of transgenic plants.
Images
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H2O2 Standard Curve using this assay protocol.
H2O2 Standard Curve using this assay protocol.
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Oxalate Oxidase (OxOx) activityOxalate Oxidase (OxOx) activity were measured in Barley Seed Lysate (29 µg) and Barley Sprout Lysate (60 µg). Assays were performed following kit protocol.
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Oxalate Oxidase (OxOx) activityOxalate Oxidase (OxOx) activity were measured in Barley Seed Lysate (29 µg) and Barley Sprout Lysate (60 µg). Assays were performed following kit protocol.