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Immunology Innate Immunity Chemokines Beta Chemokines (CC)

Mouse MCP-5 Antibody Pair - BSA and Azide free (ab256692)

Price and availability

670 ₸

Availability

Order now and get it on Friday March 05, 2021

Mouse MCP-5 Antibody Pair - BSA and Azide free (ab256692)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Unconjugated capture and detector antibodies
  • Adaptable to any antibody pair-based assay format
  • Antibody concentration ~ 1 mg/ml
  • BSA and azide free buffer - ready for conjugation
  • Reacts with: Mouse

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Overview

  • Product name

    Mouse MCP-5 Antibody Pair - BSA and Azide free
    See all MCP5 kits
  • Assay type

    ELISA set
  • Range

    27.56 pg/ml - 1700 pg/ml
  • Species reactivity

    Reacts with: Mouse
  • Product overview

    The Antibody Pair can be used to quantify Mouse MCP-5. BSA and Azide free antibody pairs include unconjugated capture and detector antibodies suitable for sandwich ELISAs. The antibodies are provided at an approximate concentration of 1 mg/ml as measured by the protein A280 method. The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Both capture and detector antibodies are rabbit monoclonal antibodies delivering consistent, specific, and sensitive results.


    For additional information on the performance of the antibody pair, see the equivalent SimpleStep ELISA® Kit (ab246547), which uses the same antibodies. However, due to differences in their formulation, this antibody pair cannot be used with the consumables provided with our SimpleStep ELISA Kits. Please note that the range provided for the pairs is only an estimation based on the performance of the related product using the same antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody pair in your assay.


    Download SDS here.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Reagents

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Carrier free

    Yes
  • Components 10 x 96 tests
    Mouse MCP-5 Capture Antibody (unconjugated) 1 x 100µg
    Mouse MCP-5 Detector Antibody (unconjugated) 1 x 100µg
  • Research areas

    • Immunology
    • Innate Immunity
    • Chemokines
    • Beta Chemokines (CC)
  • Function

    Chemotactic factor that attracts eosinophils, monocytes, and lymphocytes but not neutrophils. Potent monocyte active chemokine that signals through CCR2. Involved in allergic inflammation and the host response to pathogens and may play a pivotal role during early stages of allergic lung inflammation.
  • Tissue specificity

    Predominantly expressed in the lymph nodes and thymus. Also found in the salivary glands containing lymph nodes, breast, heart, lung, brain, small intestine, kidney and colon.
  • Sequence similarities

    Belongs to the intercrine beta (chemokine CC) family.
  • Cellular localization

    Secreted.
  • Target information above from: UniProt accession Q62401 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Alternative names

    • C-C motif chemokine 12
    • Ccl12
    • CCL12_MOUSE
    • Macrophage/monocyte Chemotactic Protein
    • MCP-1-related chemokine
    • MCP-5
    • Monocyte chemoattractant protein 5
    • Monocyte chemotactic protein 5
    • OTTMUSP00000000792
    • Scya12
    • Small inducible cytokine A12
    • Small-inducible cytokine A12
    see all
  • Database links

    • Entrez Gene: 100862578 Mouse
    • Entrez Gene: 20293 Mouse
    • SwissProt: Q62401 Mouse
    • Unigene: 867 Mouse

    Images

    • Sandwich ELISA - Mouse MCP-5 Antibody Pair - BSA and Azide free (ab256692)
      Sandwich ELISA - Mouse MCP-5 Antibody Pair - BSA and Azide free (ab256692)
      To learn more about the advantages of recombinant antibodies see here.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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