Astana Biomed Group, an authorized Abcam distributor in Central Asia

Mouse MCP-5 ELISA Kit (ab246547)

Key features and details

  • One-wash 90 minute protocol
  • Sensitivity: 6 pg/ml
  • Range: 27.56 pg/ml - 1700 pg/ml
  • Sample type: Cell culture supernatant, Cit plasma, Serum
  • Detection method: Colorimetric
  • Assay type: Sandwich (quantitative)
  • Reacts with: Mouse

Overview

  • Product name

    Mouse MCP-5 ELISA Kit
    See all MCP5 kits

  • Detection method

    Colorimetric

  • Precision

    Intra-assay
    Sample n Mean SD CV%
    serum 8 3.1%
    Inter-assay
    Sample n Mean SD CV%
    serum 3 3.8%

  • Sample type

    Cell culture supernatant, Serum, Cit plasma

  • Assay type

    Sandwich (quantitative)

  • Sensitivity

    6 pg/ml

  • Range

    27.56 pg/ml - 1700 pg/ml

  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 111 106% - 114%
    Serum 116 113% - 119%
    Cit plasma 103 100% - 106%

  • Assay time

    1h 30m

  • Assay duration

    One step assay

  • Species reactivity

    Reacts with: Mouse

  • Product overview

    Mouse MCP-5 ELISA Kit (ab246547) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of MCP-5 protein in cell culture supernatant, cit plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse MCP-5 with 6 pg/ml sensitivity.


    SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:


            - Single-wash protocol reduces assay time to 90 minutes or less
            - High sensitivity, specificity and reproducibility from superior antibodies
            - Fully validated in biological samples
            - 96-wells plate breakable into 12 x 8 wells strips


    A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components 1 x 96 tests
    10X Mouse MCP-5 Capture Antibody 1 x 600µl
    10X Mouse MCP-5 Detector Antibody 1 x 600µl
    10X Wash Buffer PT (ab206977) 1 x 20ml
    Antibody Diluent 4BR 1 x 6ml
    Mouse MCP-5 Lyophilized Recombinant Protein 2 vials
    Plate Seals 1 unit
    Sample Diluent NS (ab193972) 1 x 12ml
    SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit
    Stop Solution 1 x 12ml
    TMB Development Solution 1 x 12ml

  • Research areas

  • Function

    Chemotactic factor that attracts eosinophils, monocytes, and lymphocytes but not neutrophils. Potent monocyte active chemokine that signals through CCR2. Involved in allergic inflammation and the host response to pathogens and may play a pivotal role during early stages of allergic lung inflammation.

  • Tissue specificity

    Predominantly expressed in the lymph nodes and thymus. Also found in the salivary glands containing lymph nodes, breast, heart, lung, brain, small intestine, kidney and colon.

  • Sequence similarities

    Belongs to the intercrine beta (chemokine CC) family.

  • Cellular localization

    Secreted.
  • Information by UniProt

  • Alternative names

    • C-C motif chemokine 12
    • Ccl12
    • CCL12_MOUSE
    • Macrophage/monocyte Chemotactic Protein
    • MCP-1-related chemokine
    • MCP-5
    • Monocyte chemoattractant protein 5
    • Monocyte chemotactic protein 5
    • OTTMUSP00000000792
    • Scya12
    • Small inducible cytokine A12
    • Small-inducible cytokine A12
    see all

  • Database links

    Images

    • Other - Mouse MCP-5 ELISA Kit (ab246547)
      Other - Mouse MCP-5 ELISA Kit (ab246547)

      SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

       

    • Example of mouse MCP-5 standard curve in Sample Diluent NS.
      Example of mouse MCP-5 standard curve in Sample Diluent NS.

      The MCP-5 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

    • Interpolated concentrations of spiked MCP-5 in mouse serum, plasma (citrate) and lung supernatant samples.
      Interpolated concentrations of spiked MCP-5 in mouse serum, plasma (citrate) and lung supernatant samples.

      The concentrations of MCP-5 were measured in duplicates and interpolated from the MCP-5 standard curves. Undiluted samples are as follows: serum 50%, plasma (citrate) 50%, and lung supernatant 37.5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

       

    • Sandwich ELISA - Mouse MCP-5 ELISA Kit (ab246547)
      Sandwich ELISA - Mouse MCP-5 ELISA Kit (ab246547)
      To learn more about the advantages of recombinant antibodies see here.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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