Recombinant human IL-10 protein (Active) (ab259402)
Key features and details
- Expression system: HEK 293 cells
- Purity: >= 95% SDS-PAGE
- Endotoxin level:
- Active: Yes
- Suitable for: Functional Studies, MS, HPLC, SDS-PAGE, Cell Culture
Preparation and Storage
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Alternative names
- CSIF
- Cytokine synthesis inhibitory factor
- GVHDS
see all -
Function
Inhibits the synthesis of a number of cytokines, including IFN-gamma, IL-2, IL-3, TNF and GM-CSF produced by activated macrophages and by helper T-cells. -
Tissue specificity
Produced by a variety of cell lines, including T-cells, macrophages, mast cells and other cell types. -
Sequence similarities
Belongs to the IL-10 family. -
Cellular localization
Secreted. - Information by UniProt
Images
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Functional Studies - Recombinant human IL-10 protein (Active) (ab259402)
Fully biologically active, as determined by dose dependant stimulation of human MC/9 cells.
ED50 is ≤ 3 ng/ mL , corresponding to a specific activity of 3.33 x 105 units/mg.
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SDS-PAGE - Recombinant human IL-10 protein (Active) (ab259402)SDS-PAGE analysis of ab259402.
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HPLC - Recombinant human IL-10 protein (Active) (ab259402)Purity = 97%.
The spectrum was recorded using a 1260 Infinity II HPLC system with DAD and a MabPac RP column (3.0x100 mm, 4 µm). 5 µL of purified protein was injected and the gradient run from 80 % water:TFA (99.9:0.1 v/v) and 20 % acetonitrile:water:TFA (90:9.9:0.1 v/v/v) to 20 % water:TFA (99.9:0.1 v/v) and 80 % acetonitrile:water:TFA (90:9.9:0.1 v/v/v) within 3 minutes followed by an isocratic step for another 3 min. Flow rate was 0.5 mL/min and the column compartment temperature was 50°C.
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Mass Spectrometry - Recombinant human IL-10 protein (Active) (ab259402)M+0.17 Da (Calc. mass 17991.83 Da).
The spectrum was recorded with a 6545XT AdvanceBio LC/Q-TOF (Agilent Technologies) and a MabPac RP column (42.1x50 mm, 4 µm, Thermo Scientific). 5 µL of purified protein was injected and the gradient run from 85 % water:FA (99.9:0.1 v/v) and 15 % acetonitrile:FA (90:9.9:0.1 v/v/v) to 55 % water:FA (99.9:0.1 v/v) and 45 % acetonitrile:FA (90:9.9:0.1 v/v/v) within 3 minutes followed by an isocratic step for another 2.5 min. Flow rate was 0.4 mL/min and the column compartment temperature was 60°C. Data was analysed and deconvoluted using the Bioconfirm software (Agilent Technologies).
