Rabbit IL-17A ELISA Kit (ab273240)
Key features and details
- Sensitivity: 0.2 ng/ml
- Range: 0.205 ng/ml - 50 ng/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Rabbit
Overview
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Product name
Rabbit IL-17A ELISA Kit
See all IL-17A kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall Inter-assay Sample n Mean SD CV% Overall -
Sample type
Cell culture supernatant, Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
0.2 ng/ml -
Range
0.205 ng/ml - 50 ng/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 118 96% - 145% Plasma 116.1 85% - 146% Cell culture media 130.5 116% - 143% -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Rabbit -
Product overview
Rabbit IL-17A ELISA Kit (ab273240) is an in-vitro enzyme-linked immunosorbent assay for the quantitative measurement of rabbit IL-17A in serum (rabbit IL-17A concentration is low in normal serum and plasma samples, and may not be detectable in this assay), plasma and cell culture supernatants.
This assay employs an antibody specific for rabbit IL-17A coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-17A present in a sample is bound to the wells by the immobilized antibody. The wells are washed, and biotinylated anti- rabbit IL-17A antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-17A bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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Tested applications
Suitable for: Sandwich ELISAmore details -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 20X Wash Buffer 1 x 25ml 350X HRP-Streptavidin Concentrate 1 x 200µl 5X Assay Diluent 1 x 15ml Anti-Rabbit IL-17A coated Microplate (12 x 8 wells) 1 unit Biotinylated Anti-Rabbit IL-17A Antibody 2 vials Rabbit IL-17A Standard (Lyophilized) 2 vials Stop Solution 1 x 8ml TMB Substrate Solution 1 x 12ml -
Research areas
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Function
Induces stromal cells to produce proinflammatory and hematopoietic cytokines. Enhances the surface expression of the intracellular adhesion molecule-1 (ICAM-1) in fibroblasts. -
Tissue specificity
Restricted to activated memory T-cells. -
Sequence similarities
Belongs to the IL-17 family. -
Post-translational
modificationsFound both in glycosylated and nonglycosylated forms. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- CTLA 8
- CTLA-8
- CTLA8
see all -
Database links
- Entrez Gene: 100339322 Rabbit
Images
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Example data
This standard curve is for demonstration only. A standard curve must be run with each assay.