Mouse Eotaxin-2 Matched Antibody Pair Kit (ab213460)
Key features and details
- Unlabeled capture antibody, biotin-labeled detection antibody and calibrated protein standard
- For economical ELISA and ELISA-based assay development
- Reacts with: Mouse
- Range: 7.8 pg/ml - 500 pg/ml
Overview
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Product name
Mouse Eotaxin-2 Matched Antibody Pair Kit
See all Eotaxin 2 kits -
Detection method
Colorimetric -
Assay type
ELISA set -
Sensitivity
4 pg/ml -
Range
7.8 pg/ml - 500 pg/ml -
Species reactivity
Reacts with: Mouse -
Product overview
Mouse Eotaxin-2 Matched Antibody Pair Kits include a capture and a biotinylated detector antibody pair, along with a calibrated protein standard, suitable for sandwich ELISA. The Matched Antibody Pair Kit can be used to quantify native and recombinant mouse Eotaxin-2.
Optimization of the kit reagents to sample type, immunoassay format or instrumentation may be required. Guidelines for use of this kit in a standard 96-well microplate sandwich ELISA using HRP/TMB system of colorimetric detection is described in this assay procedure for the purposes of quantification.
Protocol information and tips on the use of the Matched Antibody Pair kits for sandwich ELISA can be found on our website. An accessory pack can be purchased which includes buffer reagents required to perform 10 x 96-well plate sandwich ELISAs (ab210905).
For additional information on the performance of the antibody pair used in this kit, please see our equivalent SimpleStep ELISA kit ab206985. Please note that while the antibody pair is the same provided in the corresponding SimpleStep ELISA Kit, due to differences in their formulation, this antibody pair cannot be used with the consumables provided with our SimpleStep ELISA Kits.
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Tested applications
Suitable for: ELISA, IAmore details -
Platform
Reagents
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 10 x 96 tests 5 x 96 tests Mouse Eotaxin 2 Capture Antibody 2 x 50µg 1 x 50µg Mouse Eotaxin 2 Detector Antibody 2 x 12.5µg 1 x 12.5µg Mouse Eotaxin 2 Lyophilized Protein 2 vials 1 vial -
Research areas
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Function
Chemotactic for resting T-lymphocytes, and eosinophils. Has lower chemotactic activity for neutrophils but none for monocytes and activated lymphocytes. Is a strong suppressor of colony formation by a multipotential hematopoietic progenitor cell line. Binds to CCR3. -
Tissue specificity
Activated monocytes and activated T lymphocytes. -
Sequence similarities
Belongs to the intercrine beta (chemokine CC) family. -
Post-translational
modificationsN-glycosylated. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- C C motif chemokine 24
- C-C motif chemokine 24
- CCL24
see all -
Database links
- Entrez Gene: 56221 Mouse
- SwissProt: Q9JKC0 Mouse
- Unigene: 31505 Mouse
Images
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Mouse Eotaxin-2 standard curve.Standard calibration curve. Background subtracted values are graphed.
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Sandwich ELISA - Mouse Eotaxin-2 Matched Antibody Pair Kit (ab213460)To learn more about the advantages of recombinant antibodies see here.
