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Mouse Eotaxin ELISA Kit (CCL11) (ab201277)

Key features and details

  • One-wash 90 minute protocol
  • Sensitivity: 8.1 pg/ml
  • Range: 23.44 pg/ml - 1500 pg/ml
  • Sample type: Cell culture supernatant, EDTA Plasma, Serum
  • Detection method: Colorimetric
  • Assay type: Sandwich (quantitative)
  • Reacts with: Mouse

Overview

  • Product name

    Mouse Eotaxin ELISA Kit (CCL11)
    See all Eotaxin kits

  • Detection method

    Colorimetric

  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Overall 5 7.3%
    Inter-assay
    Sample n Mean SD CV%
    Overall 3 8.5%

  • Sample type

    Cell culture supernatant, Serum, EDTA Plasma

  • Assay type

    Sandwich (quantitative)

  • Sensitivity

    8.1 pg/ml

  • Range

    23.44 pg/ml - 1500 pg/ml

  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Serum 90 84% - 96%
    Cell culture media 99 99% - 100%
    EDTA Plasma 108 99% - 107%

  • Assay time

    1h 30m

  • Assay duration

    One step assay

  • Species reactivity

    Reacts with: Mouse

  • Product overview

    Mouse Eotaxin ELISA Kit (CCL11) (ab201277) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Eotaxin (CCL11) protein in cell culture supernatant, edta plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse Eotaxin (CCL11) with 8.1 pg/ml sensitivity.


    SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:


            - Single-wash protocol reduces assay time to 90 minutes or less
            - High sensitivity, specificity and reproducibility from superior antibodies
            - Fully validated in biological samples
            - 96-wells plate breakable into 12 x 8 wells strips


    A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

  • Notes

    Mouse Eotaxin (CCL11) is a 8.4 kDa cytokine, constitutively expressed in the thymus and inducibly expressed in the lung, intestine, heart, spleen and kidney. Eotaxin is a chemoattractant for eosinophils during inflammation and allergic reactions, and is also involved in mast cell differentiation and growth of myeloid cell progenitors.

  • Platform

    Microplate (12 x 8 well strips)

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components 1 x 96 tests
    10X Mouse Eotaxin Capture Antibody 1 x 600µl
    10X Mouse Eotaxin Detector Antibody 1 x 600µl
    10X Wash Buffer PT (ab206977) 1 x 20ml
    Antibody Diluent 4BI 1 x 6ml
    Mouse Eotaxin Recombinant Lyophilized Protein 2 vials
    Plate Seals 1 unit
    Sample Diluent NS (ab193972) 1 x 50ml
    SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit
    Stop Solution 1 x 12ml
    TMB Development Solution 1 x 12ml

  • Research areas

  • Function

    In response to the presence of allergens, this protein directly promotes the accumulation of eosinophils, a prominent feature of allergic inflammatory reactions. Binds to CCR3.

  • Sequence similarities

    Belongs to the intercrine beta (chemokine CC) family.

  • Post-translational
    modifications

    O-linked glycan consists of a Gal-GalNAc disaccharide which is mofified with up to 2 sialic acid residues.

  • Cellular localization

    Secreted.
  • Information by UniProt

  • Alternative names

    • C-C motif chemokine 11
    • CCL 11
    • CCL11
    • CCL11_HUMAN
    • chemokine (C-C motif) ligand 11
    • Chemokine C C motif ligand 11
    • Chemokine CC Motif Ligand 11
    • Chemokine ligand 11
    • Eosinophil chemotactic protein
    • Eotaxin
    • eotaxin-1
    • MGC22554
    • SCYA 11
    • SCYA11
    • Small inducible cytokine A11
    • Small inducible cytokine subfamily A (Cys Cys) member 11
    • small inducible cytokine subfamily A (Cys-Cys), member 11 (eotaxin)
    • Small inducible cytokine subfamily A member 11
    • Small-inducible cytokine A11
    see all

  • Database links

    Images

    • Other - Mouse Eotaxin ELISA Kit (CCL11) (ab201277)
      Other - Mouse Eotaxin ELISA Kit (CCL11) (ab201277)

      SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

       

    • Example of Eotaxin standard curve.
      Example of Eotaxin standard curve.

      Background-subtracted data values (mean +/- SD) are graphed.

    • Linearity of dilution of the assay. Interpolated concentrations of Eotaxin in mouse serum, plasma (EDTA) and culture media.
      Linearity of dilution of the assay. Interpolated concentrations of Eotaxin in mouse serum, plasma (EDTA) and culture media.

      The concentrations of Eotaxin were measured in duplicate and interpolated from the Eotaxin standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Eotaxin concentration was determined to be 484 pg/mL in mouse serum and 668 pg/mL in mouse plasma.

    • Sandwich ELISA - Mouse Eotaxin ELISA Kit (CCL11) (ab201277)
      Sandwich ELISA - Mouse Eotaxin ELISA Kit (CCL11) (ab201277)
      To learn more about the advantages of recombinant antibodies see here.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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