Mouse Eotaxin ELISA Kit (CCL11) (ab201277)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 8.1 pg/ml
- Range: 23.44 pg/ml - 1500 pg/ml
- Sample type: Cell culture supernatant, EDTA Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse
Overview
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Product name
Mouse Eotaxin ELISA Kit (CCL11)
See all Eotaxin kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall 5 7.3% Inter-assay Sample n Mean SD CV% Overall 3 8.5% -
Sample type
Cell culture supernatant, Serum, EDTA Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
8.1 pg/ml -
Range
23.44 pg/ml - 1500 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 90 84% - 96% Cell culture media 99 99% - 100% EDTA Plasma 108 99% - 107% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Mouse -
Product overview
Mouse Eotaxin ELISA Kit (CCL11) (ab201277) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Eotaxin (CCL11) protein in cell culture supernatant, edta plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Mouse Eotaxin (CCL11) with 8.1 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
Mouse Eotaxin (CCL11) is a 8.4 kDa cytokine, constitutively expressed in the thymus and inducibly expressed in the lung, intestine, heart, spleen and kidney. Eotaxin is a chemoattractant for eosinophils during inflammation and allergic reactions, and is also involved in mast cell differentiation and growth of myeloid cell progenitors.
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Platform
Microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Mouse Eotaxin Capture Antibody 1 x 600µl 10X Mouse Eotaxin Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml Antibody Diluent 4BI 1 x 6ml Mouse Eotaxin Recombinant Lyophilized Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
In response to the presence of allergens, this protein directly promotes the accumulation of eosinophils, a prominent feature of allergic inflammatory reactions. Binds to CCR3. -
Sequence similarities
Belongs to the intercrine beta (chemokine CC) family. -
Post-translational
modificationsO-linked glycan consists of a Gal-GalNAc disaccharide which is mofified with up to 2 sialic acid residues. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- C-C motif chemokine 11
- CCL 11
- CCL11
see all -
Database links
- Entrez Gene: 20292 Mouse
- SwissProt: P48298 Mouse
- Unigene: 4686 Mouse
Images
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Other - Mouse Eotaxin ELISA Kit (CCL11) (ab201277)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of Eotaxin standard curve.Background-subtracted data values (mean +/- SD) are graphed.
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Linearity of dilution of the assay. Interpolated concentrations of Eotaxin in mouse serum, plasma (EDTA) and culture media.The concentrations of Eotaxin were measured in duplicate and interpolated from the Eotaxin standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Eotaxin concentration was determined to be 484 pg/mL in mouse serum and 668 pg/mL in mouse plasma.
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Sandwich ELISA - Mouse Eotaxin ELISA Kit (CCL11) (ab201277)To learn more about the advantages of recombinant antibodies see here.
