Mouse Eotaxin Matched Antibody Pair Kit (ab212068)
Key features and details
- Unlabeled capture antibody, biotin-labeled detection antibody and calibrated protein standard
- For economical ELISA and ELISA-based assay development
- Reacts with: Mouse
- Range: 3.9 pg/ml - 250 pg/ml
Overview
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Product name
Mouse Eotaxin Matched Antibody Pair Kit
See all Eotaxin kits -
Detection method
Colorimetric -
Assay type
ELISA set -
Sensitivity
0.5 pg/ml -
Range
3.9 pg/ml - 250 pg/ml -
Species reactivity
Reacts with: Mouse -
Product overview
Mouse Eotaxin Matched Antibody Pair Kits include a capture and a biotinylated detector antibody pair, along with a calibrated protein standard, suitable for sandwich ELISA. The Matched Antibody Pair Kit can be used to quantify native and recombinant mouse Eotaxin.
Optimization of the kit reagents to sample type, immunoassay format or instrumentation may be required. Guidelines for use of this kit in a standard 96-well microplate sandwich ELISA using HRP/TMB system of colorimetric detection is described in this assay procedure for the purposes of quantification.
Protocol information and tips on the use of the Matched Antibody Pair kits for sandwich ELISA can be found on our website. An accessory pack can be purchased which includes buffer reagents required to perform 10 x 96-well plate sandwich ELISAs (ab210905).
For additional information on the performance of the antibody pair used in this kit, please see our equivalent SimpleStep ELISA kit ab210277. Please note that while the antibody pair is the same provided in the corresponding SimpleStep ELISA Kit, due to differences in their formulation, this antibody pair cannot be used with the consumables provided with our SimpleStep ELISA Kits.
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Tested applications
Suitable for: ELISA, IAmore details -
Platform
Reagents
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 10 x 96 tests 5 x 96 tests Mouse Eotaxin Capture Antibody 2 x 50µg 1 x 50µg Mouse Eotaxin Detector Antibody 2 x 12.5µg 1 x 12.5µg Mouse Eotaxin Lyophilized Protein 2 vials 1 vial -
Research areas
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Function
In response to the presence of allergens, this protein directly promotes the accumulation of eosinophils, a prominent feature of allergic inflammatory reactions. Binds to CCR3. -
Sequence similarities
Belongs to the intercrine beta (chemokine CC) family. -
Post-translational
modificationsO-linked glycan consists of a Gal-GalNAc disaccharide which is mofified with up to 2 sialic acid residues. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- C-C motif chemokine 11
- CCL 11
- CCL11
see all -
Database links
- Entrez Gene: 20292 Mouse
- SwissProt: P48298 Mouse
- Unigene: 4686 Mouse
Images
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Mouse Eotaxin standard curve
Standard calibration curve. Background subtracted values are graphed.
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Sandwich ELISA - Mouse Eotaxin Matched Antibody Pair Kit (ab212068)To learn more about the advantages of recombinant antibodies see here.
