Human RANTES ELISA Kit (ab100633)
Key features and details
- Sensitivity: 3 pg/ml
- Range: 2.74 pg/ml - 2000 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 20X Wash Buffer Concentrate 1 x 25ml 300X HRP-Streptavidin Concentrate 1 x 200µl 5X Assay Diluent B 1 x 15ml Assay Diluent A 1 x 30ml Biotinylated anti-Human RANTES 2 vials RANTES Microplate (12 x 8 wells) 1 unit Recombinant Human RANTES Standard (lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
Research areas
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Function
Chemoattractant for blood monocytes, memory T-helper cells and eosinophils. Causes the release of histamine from basophils and activates eosinophils. Binds to CCR1, CCR3, CCR4 and CCR5. One of the major HIV-suppressive factors produced by CD8+ T-cells. Recombinant RANTES protein induces a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV). The processed form RANTES(3-68) acts as a natural chemotaxis inhibitor and is a more potent inhibitor of HIV-1-infection. The second processed form RANTES(4-68) exhibits reduced chemotactic and HIV-suppressive activity compared with RANTES(1-68) and RANTES(3-68) and is generated by an unidentified enzyme associated with monocytes and neutrophils. -
Tissue specificity
T-cell and macrophage specific. -
Sequence similarities
Belongs to the intercrine beta (chemokine CC) family. -
Post-translational
modificationsN-terminal processed form RANTES(3-68) is produced by proteolytic cleavage, probably by DPP4, after secretion from peripheral blood leukocytes and cultured sarcoma cells.
The identity of the O-linked saccharides at Ser-27 and Ser-28 are not reported in PubMed:1380064. They are assigned by similarity. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- Beta chemokine RANTES
- Beta chemokine RANTES precursor
- C C motif chemokine 5
see all -
Database links
- Entrez Gene: 6352 Human
- Omim: 187011 Human
- SwissProt: P13501 Human
- Unigene: 514821 Human
Images
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RANTES Human ELISA Kit (ab100633)
Standard curve: mean of duplicates (+/- SD) with background reads subtracted
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RANTES Human ELISA Kit (ab100633)Standard curve: mean of duplicates (+/- SD) with background reads subtracted
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RANTES Human ELISA Kit (ab100633)RANTES measured in cell supernatants showing quantity (pg) per mL of tested sample. Samples were diluted 1-8 fold.
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RANTES Human ELISA Kit (ab100633)RANTES measured in biological fluids showing quantity (pg) per mL of tested sample. Samples were diluted 6-54 fold.
