Anti-CCL27 antibody [EPR17284-217] - BSA and Azide free (ab228323)
![Anti-CCL27 antibody [EPR17284-217] - BSA and Azide free (ab228323)](https://www.abcam.com/ps/products/228/ab228323/Images/ab228323-2-ab181323291658181323IP.jpg "Anti-CCL27 antibody [EPR17284-217] - BSA and Azide free (ab228323)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR17284-217] to CCL27 - BSA and Azide free
- Suitable for: WB, IP
- Reacts with: Mouse
Overview
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Product name
Anti-CCL27 antibody [EPR17284-217] - BSA and Azide free
See all CCL27 primary antibodies -
Description
Rabbit monoclonal [EPR17284-217] to CCL27 - BSA and Azide free -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP MouseWB Mouse
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Immunogen
Recombinant full length protein. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Mouse skin lysate.
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General notes
Ab228323 is the carrier-free version of ab181323. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab228323 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR17284-217 -
Isotype
IgG -
Research areas
Images
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Immunoprecipitation - Anti-CCL27 antibody [EPR17284-217] - BSA and Azide free (ab228323)
CCL27 was immunoprecipitated from 0.35 mg of mouse skin lysate with ab181323 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab181323 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.
Lane 1: Mouse skin lysate 10 µg (Input).
Lane 2: ab181323 IP in mouse skin lysate (+).
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab181323 in mouse skin lysate (-).
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 3 minutes.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab181323).
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![Western blot - Anti-CCL27 antibody [EPR17284-217] - BSA and Azide free (ab228323)](https://www.abcam.com/ps/products/228/ab228323/Images/ab228323-291668-228323WB.jpg)
Western blot - Anti-CCL27 antibody [EPR17284-217] - BSA and Azide free (ab228323)Anti-CCL27 antibody [EPR17284-217] (ab181323) at 1/1000 dilution + Mouse skin lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Developed using the ECL technique.
Predicted band size: 13 kDa
Observed band size: 13 kDa
Exposure time: 5 secondsBlocking/Dilution buffer: 5% NFDM/TBST.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab181323).
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![Anti-CCL27 antibody [EPR17284-217] - BSA and Azide free (ab228323)](https://www.abcam.com/ps/products/228/ab228323/Images/ab228323-3-benefits-of-recombinant-antibodies.png)
Anti-CCL27 antibody [EPR17284-217] - BSA and Azide free (ab228323)
