Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-CCL27 antibody [EPR17284-217] (ab181323)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR17284-217] to CCL27
  • Suitable for: WB, IP
  • Reacts with: Mouse

Overview

  • Product name

    Anti-CCL27 antibody [EPR17284-217]
    See all CCL27 primary antibodies

  • Description

    Rabbit monoclonal [EPR17284-217] to CCL27

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IP
    Mouse
    WB
    Mouse
    See all applications and species data

  • Immunogen

    Recombinant full length protein. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Mouse skin lysate. IP: Mouse skin lysate.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR17284-217

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-CCL27 antibody [EPR17284-217] (ab181323)
    Western blot - Anti-CCL27 antibody [EPR17284-217] (ab181323)
    Anti-CCL27 antibody [EPR17284-217] (ab181323) at 1/1000 dilution + Mouse skin lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Developed using the ECL technique.

    Predicted band size: 13 kDa
    Observed band size: 13 kDa


    Exposure time: 5 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Immunoprecipitation - Anti-CCL27 antibody [EPR17284-217] (ab181323)
    Immunoprecipitation - Anti-CCL27 antibody [EPR17284-217] (ab181323)

    CCL27 was immunoprecipitated from 0.35 mg of mouse skin lysate with ab181323 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab181323 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

    Lane 1: Mouse skin lysate 10 µg (Input). 

    Lane 2: ab181323 IP in mouse skin lysate (+). 

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab181323 in mouse skin lysate (-).

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 3 minutes.

  • Anti-CCL27 antibody [EPR17284-217] (ab181323)
    Anti-CCL27 antibody [EPR17284-217] (ab181323)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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