TSC-1 overexpression 293T lysate (whole cell) (ab94221)
Overview
-
Product name
TSC-1 overexpression 293T lysate (whole cell) -
General notes
ab94221 is a 293T cell transfected lysate in which Human TSC-1 has been transiently over-expressed using a pCMV-TSC-1 plasmid. The lysate is provided in 1X Sample Buffer. Note: For more detailed how the transfected lysate was prepared view preparation notes
-
Tested applications
Suitable for: WBmore details
Properties
-
Mycoplasma free
Yes -
Form
Liquid -
Storage instructions
Shipped on dry ice. Upon delivery aliquot and store at -20ºC. Avoid freeze / thaw cycles. -
Storage buffer
Constituents: 0.01% Bromophenol blue, 2.3% Beta mercaptoethanol, 2% Sodium lauryl sulfate, 0.788% Tris HCl, 10% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Research areas
-
Background
Function: Chemotactic for eosinophils and basophils. Binds to CCR3. Tissue specificity: Ubiquitously expressed at low levels in various tissues including heart and ovary. Similarity: Belongs to the intercrine beta (chemokine CC) family.
Images
-
SDS-PAGE - TSC-1 overexpression 293T lysate (whole cell) (ab94221)ab94221 at 15µg/lane on an SDS-PAGE gel.
-
Western blot - TSC-1 overexpression 293T lysate (whole cell) (ab94221)All lanes : Anti-TSC-1 antibody (ab67260) at 1/500 dilution
Lane 1 :TSC-1 overexpression 293T lysate (whole cell) (ab94221)
Lane 2 : 293T non-transfected lysate
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution
