Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Eotaxin antibody [EPR5825] (ab133604)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR5825] to Eotaxin
  • Suitable for: WB, IHC-P
  • Reacts with: Human, Recombinant fragment

Overview

  • Product name

    Anti-Eotaxin antibody [EPR5825]
    See all Eotaxin primary antibodies

  • Description

    Rabbit monoclonal [EPR5825] to Eotaxin

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Recombinant fragment
    See all applications and species data

  • Immunogen

    Synthetic peptide corresponding to a region within Human Eotaxin

  • Positive control

    • Eotaxin recombinant protein; Human lung tissue

  • General notes

     

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Stable for 12 months at -20°C.

  • Dissociation constant (KD)

    KD = 7.10 x 10 -11 M
    Learn more about KD

  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 0.05% BSA, 59% PBS, 40% Glycerol (glycerin, glycerine)
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR5825

  • Isotype

    IgG

  • Research areas

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Eotaxin antibody [EPR5825] (ab133604)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Eotaxin antibody [EPR5825] (ab133604)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human pancreatic carcinoma tissue sections labeling Eotaxin with Purified ab133604 at 1:100 dilution (1.21 μg/ml). Heat mediated antigen retrieval was performed using EDTA Buffer, pH9.0. Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control.

  • Western blot - Anti-Eotaxin antibody [EPR5825] (ab133604)
    Western blot - Anti-Eotaxin antibody [EPR5825] (ab133604)
    Anti-Eotaxin antibody [EPR5825] (ab133604) at 1/10000 dilution (purified) + Human Eotaxin recombinant protein lysates at 0.015 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 11 kDa
    Observed band size: 8 kDa
    why is the actual band size different from the predicted?



    Blocking and diluting buffer: 5% NFDM/TBST

    Recombinant protein source: Peprotech Inc.
    The region it covers: aa24-aa97(8.3kDa)
    Tag info: N/A

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Eotaxin antibody [EPR5825] (ab133604)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Eotaxin antibody [EPR5825] (ab133604)

    Immunohistochemical analysis of paraffin-embedded Human lung tissue labelling Eotaxin with unpurified ab133604 at 1/1000.

    Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.

  • Western blot - Anti-Eotaxin antibody [EPR5825] (ab133604)
    Western blot - Anti-Eotaxin antibody [EPR5825] (ab133604)
    Anti-Eotaxin antibody [EPR5825] (ab133604) at 1/1000 dilution (unpurified) + Eotaxin recombinant protein at 0.01 µg

    Secondary
    Goat anti-Rabbit HRP at 1/2000 dilution

    Predicted band size: 11 kDa
    Observed band size: 8 kDa why is the actual band size different from the predicted?

  • OI-RD Scanning - Anti-Eotaxin antibody [EPR5825] (ab133604)
    OI-RD Scanning - Anti-Eotaxin antibody [EPR5825] (ab133604)
    Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD
  • Anti-Eotaxin antibody [EPR5825] (ab133604)
    Anti-Eotaxin antibody [EPR5825] (ab133604)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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