Antibodies, Proteins, Kits and Reagents for Life Science

284 784 ₸ Product size

100 µl 284 784 ₸

Order now and get it on Wednesday March 03, 2021

Anti-CCL27 antibody [EPR8582(2)] (ab186421)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR8582(2)] to CCL27
Suitable for: WB, ICC/IF, Flow Cyt
Reacts with: Human

Product name

Anti-CCL27 antibody [EPR8582(2)]
See all CCL27 primary antibodies
Description

Rabbit monoclonal [EPR8582(2)] to CCL27
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC/IF	Human
WB	Human

See all applications and species data

Immunogen

This information is proprietary to Abcam and/or its suppliers.
Positive control
- Human CCL27 recombinant protein; A431 and HACAT cells.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR8582(2)
Isotype

IgG
Research areas

Immunocytochemistry/ Immunofluorescence - Anti-CCL27 antibody [EPR8582(2)] (ab186421)

Immunofluorescent analysis of 4% paraformaldehyde-fixed A431 cells labeling CCL27 with ab186421 at 1/250 dilution followed by Goat anti rabbit IgG (Alexa Fluor® 555) secondary antibody at 1/200 dilution. Counter stained with Dapi.
Western blot - Anti-CCL27 antibody [EPR8582(2)] (ab186421)

Anti-CCL27 antibody [EPR8582(2)] (ab186421) at 1/5000 dilution + Human CCL27 recombinant protein at 0.01 µg

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Predicted band size: 13 kDa
Flow Cytometry - Anti-CCL27 antibody [EPR8582(2)] (ab186421)

Flow cytometric analysis of 2% paraformaldehyde-fixed HACAT cells labeling CCL27 with ab186421 at 1/320 dilution (red) compared to a Rabbit monoclonal IgG isotype control (green), followed by Goat anti rabbit IgG (FITC) secondary antibody at 1/150 dilution.
Anti-CCL27 antibody [EPR8582(2)] (ab186421)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com