Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Eotaxin antibody [EPR5825] - BSA and Azide free (ab226143)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR5825] to Eotaxin - BSA and Azide free
  • Suitable for: WB, IHC-P
  • Reacts with: Human

Overview

  • Product name

    Anti-Eotaxin antibody [EPR5825] - BSA and Azide free
    See all Eotaxin primary antibodies

  • Description

    Rabbit monoclonal [EPR5825] to Eotaxin - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: IP

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Eotaxin recombinant protein; Human lung tissue

  • General notes

    ab226143 is the carrier-free version of ab133604 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab226143 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.

  • Dissociation constant (KD)

    KD = 7.10 x 10 -11 M
    Learn more about KD

  • Storage buffer

    pH: 7.2
    Constituent: PBS

  • Carrier free

    Yes
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR5825

  • Isotype

    IgG

  • Research areas

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Eotaxin antibody [EPR5825] - BSA and Azide free (ab226143)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Eotaxin antibody [EPR5825] - BSA and Azide free (ab226143)
    This data was developed using ab133604, the same antibody clone in a different buffer formulation.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human pancreatic carcinoma tissue sections labeling Eotaxin with Purified ab133604 at 1:100 dilution (1.21 μg/ml). Heat mediated antigen retrieval was performed using EDTA Buffer, pH9.0. Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control.
  • Western blot - Anti-Eotaxin antibody [EPR5825] - BSA and Azide free (ab226143)
    Western blot - Anti-Eotaxin antibody [EPR5825] - BSA and Azide free (ab226143)
    Anti-Eotaxin antibody [EPR5825] (ab133604) at 1/10000 dilution (purified) + Human Eotaxin recombinant protein at 0.015 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 11 kDa
    Observed band size: 8 kDa
    why is the actual band size different from the predicted?



    This data was developed using ab133604, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    Recombinant protein source: Peprotech Inc. The region it covers: aa24-aa97(8.3kDa).  Tag info: N/A.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Eotaxin antibody [EPR5825] - BSA and Azide free (ab226143)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Eotaxin antibody [EPR5825] - BSA and Azide free (ab226143)
    This data was developed using ab133604, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human lung tissue labelling Eotaxin with unpurified ab133604 at 1/1000. Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.
  • Western blot - Anti-Eotaxin antibody [EPR5825] - BSA and Azide free (ab226143)
    Western blot - Anti-Eotaxin antibody [EPR5825] - BSA and Azide free (ab226143)
    Anti-Eotaxin antibody [EPR5825] (ab133604) at 1/1000 dilution (unpurified) + Eotaxin recombinant protein at 0.01 µg

    Secondary
    Goat anti-Rabbit HRP at 1/2000 dilution

    Predicted band size: 11 kDa
    Observed band size: 8 kDa why is the actual band size different from the predicted?



    This data was developed using ab133604, the same antibody clone in a different buffer formulation.

  • OI-RD Scanning - Anti-Eotaxin antibody [EPR5825] - BSA and Azide free (ab226143)
    OI-RD Scanning - Anti-Eotaxin antibody [EPR5825] - BSA and Azide free (ab226143)
    This data was developed using ab133604, the same antibody clone in a different buffer formulation.Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD
  • Anti-Eotaxin antibody [EPR5825] - BSA and Azide free (ab226143)
    Anti-Eotaxin antibody [EPR5825] - BSA and Azide free (ab226143)

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