Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Vinculin antibody [SP117] - BSA and Azide free (ab240945)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [SP117] to Vinculin - BSA and Azide free
  • Suitable for: IHC-P
  • Reacts with: Human

Overview

  • Product name

    Anti-Vinculin antibody [SP117] - BSA and Azide free
    See all Vinculin primary antibodies

  • Description

    Rabbit monoclonal [SP117] to Vinculin - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Human heart, cerebellum, skeletal muscle, prostate, colon, small intestine, stomach, bladder, breast and breast adenocarcinoma tissue, rat colon.

  • General notes

    FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.

    Ab240945 is the carrier-free version of ab111004. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab240945 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vinculin antibody [SP117] - BSA and Azide free (ab240945)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vinculin antibody [SP117] - BSA and Azide free (ab240945)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Rat colon tissue sections labeling Vinculin with ab111004 at 1/100 dilution (1.84 μg/ml). Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 10mins. Goat Anti-Rabbit & Mouse IgG (HRP) was used as the secondary antibody. Hematoxylin was used as a counterstain. Positive staining on the smooth muscle in rat colon, performed on a Leica Biosystems BOND™ RX instrument.
    The section was incubated with ab111004 for 10 mins at room temperature.

    This image was generated using ab111004, the same clone, but with a different buffer formulation.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vinculin antibody [SP117] - BSA and Azide free (ab240945)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vinculin antibody [SP117] - BSA and Azide free (ab240945)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human colon tissue sections labeling Vinculin with ab111004 at 1/100 dilution (1.84 μg/ml). Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 10mins. Goat Anti-Rabbit & Mouse IgG (HRP) was used as the secondary antibody. Hematoxylin was used as a counterstain. Positive staining on the smooth muscle in human colon, performed on a Leica Biosystems BOND™ RX instrument.
    The section was incubated with ab111004 for 10 mins at room temperature.
    This image was generated using ab111004, the same clone, but with a different buffer formulation.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vinculin antibody [SP117] - BSA and Azide free (ab240945)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vinculin antibody [SP117] - BSA and Azide free (ab240945)

    Formalin-fixed, paraffin-embedded human heart tissue stained for Vinculin using ab111004 at 1/100 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab111004).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vinculin antibody [SP117] - BSA and Azide free (ab240945)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vinculin antibody [SP117] - BSA and Azide free (ab240945)

    Formalin-fixed, paraffin-embedded human breast adenocarcinoma tissue stained for Vinculin using ab111004 at 1/100 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab111004).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vinculin antibody [SP117] - BSA and Azide free (ab240945)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vinculin antibody [SP117] - BSA and Azide free (ab240945)

    Formalin-fixed, paraffin-embedded human breast tissue stained for Vinculin using ab111004 at 1/100 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab111004).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vinculin antibody [SP117] - BSA and Azide free (ab240945)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vinculin antibody [SP117] - BSA and Azide free (ab240945)

    Formalin-fixed, paraffin-embedded human bladder tissue stained for Vinculin using ab111004 at 1/100 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab111004).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vinculin antibody [SP117] - BSA and Azide free (ab240945)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vinculin antibody [SP117] - BSA and Azide free (ab240945)

    Formalin-fixed, paraffin-embedded human stomach tissue stained for Vinculin using ab111004 at 1/100 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab111004).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vinculin antibody [SP117] - BSA and Azide free (ab240945)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vinculin antibody [SP117] - BSA and Azide free (ab240945)

    Formalin-fixed, paraffin-embedded human small intestine tissue stained for Vinculin using ab111004 at 1/100 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab111004).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vinculin antibody [SP117] - BSA and Azide free (ab240945)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vinculin antibody [SP117] - BSA and Azide free (ab240945)

    Formalin-fixed, paraffin-embedded human colon tissue stained for Vinculin using ab111004 at 1/100 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab111004).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vinculin antibody [SP117] - BSA and Azide free (ab240945)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vinculin antibody [SP117] - BSA and Azide free (ab240945)

    Formalin-fixed, paraffin-embedded human prostate tissue stained for Vinculin using ab111004 at 1/100 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab111004).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vinculin antibody [SP117] - BSA and Azide free (ab240945)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vinculin antibody [SP117] - BSA and Azide free (ab240945)

    Formalin-fixed, paraffin-embedded human skeletal muscle tissue stained for Vinculin using ab111004 at 1/100 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab111004).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vinculin antibody [SP117] - BSA and Azide free (ab240945)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Vinculin antibody [SP117] - BSA and Azide free (ab240945)

    Formalin-fixed, paraffin-embedded human cerebellum tissue stained for Vinculin using ab111004 at 1/100 dilution in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and sodium azide (ab111004).

  • Anti-Vinculin antibody [SP117] - BSA and Azide free (ab240945)
    Anti-Vinculin antibody [SP117] - BSA and Azide free (ab240945)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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