Human Angiogenin Antibody Pair - BSA and Azide free (ab241874)
Key features and details
- Unconjugated capture and detector antibodies
- Adaptable to any antibody pair-based assay format
- Antibody concentration ~ 1 mg/ml
- BSA and azide free buffer - ready for conjugation
- Reacts with: Human
Overview
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Product name
Human Angiogenin Antibody Pair - BSA and Azide free
See all Angiogenin kits -
Assay type
ELISA set -
Range
7.8 pg/ml - 500 pg/ml -
Species reactivity
Reacts with: Human -
Product overview
The Antibody Pair can be used to quantify Human Angiogenin. BSA and Azide free antibody pairs include unconjugated capture and detector antibodies suitable for sandwich ELISAs. The antibodies are provided at an approximate concentration of 1 mg/ml as measured by the protein A280 method. The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Both capture and detector antibodies are rabbit monoclonal antibodies delivering consistent, specific, and sensitive results.
For additional information on the performance of the antibody pair, see the equivalent SimpleStep ELISA® Kit (ab219629), which uses the same antibodies. However, due to differences in their formulation, this antibody pair cannot be used with the consumables provided with our SimpleStep ELISA Kits. Please note that the range provided for the pairs is only an estimation based on the performance of the related product using the same antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody pair in your assay.
Download SDS here.
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Tested applications
Suitable for: Sandwich ELISAmore details -
Platform
Reagents
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Carrier free
Yes -
Components 10 x 96 tests Human Angiogenin Capture Antibody (unconjugated) 1 x 100µg Human Angiogenin Detector Antibody (unconjugated) 1 x 100µg -
Research areas
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Function
May function as a tRNA-specific ribonuclease that abolishes protein synthesis by specifically hydrolyzing cellular tRNAs. Binds to actin on the surface of endothelial cells; once bound, angiogenin is endocytosed and translocated to the nucleus. Angiogenin induces vascularization of normal and malignant tissues. Angiogenic activity is regulated by interaction with RNH1 in vivo. -
Tissue specificity
Expressed predominantly in the liver. Also detected in endothelial cells and spinal cord neurons. -
Involvement in disease
Defects in ANG are the cause of susceptibility to amyotrophic lateral sclerosis type 9 (ALS9) [MIM:611895]. ALS is a degenerative disorder of motor neurons in the cortex, brain stem and spinal cord. ALS is characterized by muscular weakness and atrophy. -
Sequence similarities
Belongs to the pancreatic ribonuclease family. -
Developmental stage
Low level expression in the developing fetus, increased in the neonate, and maximal in the adult. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- ALS9
- AMYOTROPHIC LATERAL SCLEROSIS
- ANG
see all -
Database links
- Entrez Gene: 283 Human
- Omim: 105850 Human
- SwissProt: P03950 Human
- Unigene: 283749 Human
Images
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Sandwich ELISA - Human Angiogenin Antibody Pair - BSA and Azide free (ab241874)To learn more about the advantages of recombinant antibodies see here.
