Human Angiogenin ELISA Kit (ab219629)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 1.06 pg/ml
- Range: 7.81 pg/ml - 500 pg/ml
- Sample type: Cell culture extracts, Cell culture media, Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human Angiogenin ELISA Kit
See all Angiogenin kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Serum 8 4.2% Inter-assay Sample n Mean SD CV% Serum 3 5.6% -
Sample type
Cell culture supernatant, Serum, Cell culture extracts, Cell culture media, Hep Plasma, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
1.06 pg/ml -
Range
7.81 pg/ml - 500 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Cell culture supernatant 110 108% - 113% Serum 103 99% - 106% Cell culture extracts 101 99% - 103% Cell culture media 104 100% - 107% Hep Plasma 103 96% - 109% EDTA Plasma 100 96% - 102% Cit plasma 101 97% - 105% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human
Does not react with: Cow -
Product overview
Human Angiogenin ELISA Kit (ab219629) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Angiogenin protein in cell culture extracts, cell culture supernatant, cit plasma, edta plasma, hep plasma, serum, and cell culture media. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Angiogenin with 1.06 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
Angiogenin is a member of the ribonuclease superfamily with approximately 35% amino acid sequence homology with pancreatic RNase. Angiogenin can act to inhibit protein synthesis by functioning as a cytotoxic tRNA-specific RNase.
Cells expressing Angiogenin include vascular endothelial and smooth muscle cells, fibroblasts, colonic epithelium, peripheral blood lymphocytes, lung cell lines, and primary gastrointestinal adenocarcinomas. Basal levels of Angiogenin are also present in normal human serum and plasma. Angiogenin has been reported to serve as protection for motor neurons and it follows that Angiogenin is associated with neurological diseases, specifically, mutations to this gene are linked to Amyotrophic Lateral Sclerosis (ALS). Human and mouse Angiogenin share 74% homology in amino acid sequence.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Wash Buffer PT (ab206977) 1 x 20ml 50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent CPI - HAMA Blocker (ab193969) 1 x 6ml 10X Human Angiogenin Capture Antibody 1 x 600µl 10X Human Angiogenin Detector Antibody 1 x 600µl Human Angiogenin Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
May function as a tRNA-specific ribonuclease that abolishes protein synthesis by specifically hydrolyzing cellular tRNAs. Binds to actin on the surface of endothelial cells; once bound, angiogenin is endocytosed and translocated to the nucleus. Angiogenin induces vascularization of normal and malignant tissues. Angiogenic activity is regulated by interaction with RNH1 in vivo. -
Tissue specificity
Expressed predominantly in the liver. Also detected in endothelial cells and spinal cord neurons. -
Involvement in disease
Defects in ANG are the cause of susceptibility to amyotrophic lateral sclerosis type 9 (ALS9) [MIM:611895]. ALS is a degenerative disorder of motor neurons in the cortex, brain stem and spinal cord. ALS is characterized by muscular weakness and atrophy. -
Sequence similarities
Belongs to the pancreatic ribonuclease family. -
Developmental stage
Low level expression in the developing fetus, increased in the neonate, and maximal in the adult. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- ALS9
- AMYOTROPHIC LATERAL SCLEROSIS
- ANG
see all -
Database links
- Entrez Gene: 283 Human
- Omim: 105850 Human
- SwissProt: P03950 Human
- Unigene: 283749 Human
Images
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SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Background-subtracted data values (mean +/- SD) are graphed.
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Background-subtracted data values (mean +/- SD) are graphed.
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The concentrations of Angiogenin were measured in duplicates, interpolated from the Angiogenin standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 1:1,000, plasma (citrate) 1:1,000, plasma (EDTA) 1:1,000, and plasma (heparin) 1:1,000. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Angiogenin concentration was determined to be 342.53 ng/mL in neat serum, 310.16 ng/mL in neat plasma (citrate), 249.90 ng/mL in neat plasma (EDTA), and 329.36 ng/mL in neat plasma (heparin).
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The concentrations of Angiogenin were measured in duplicates, interpolated from the Angiogenin standard curves and corrected for sample dilution. Undiluted samples are as follows: RPMI culture media 50% and HepG2 supernatant 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Angiogenin concentration was determined to be 527.60 pg/mL in neat, spiked RPMI culture media. The mean Angiogenin concentration was determined to be 945.93 pg/mL in neat, native HepG2 supernatant.
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The concentrations of Angiogenin were measured in duplicate and interpolated from the Angiogenin standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Angiogenin concentration was determined to be 304.03 pg/mL in HepG2 cell extract sample.
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Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Angiogenin concentration was determined to be 495.29 ng/mL with a range of 214.57 – 824.72 ng/mL.
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To learn more about the advantages of recombinant antibodies see here.