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Human Angiogenin ELISA Kit (ab219629)

Key features and details

  • One-wash 90 minute protocol
  • Sensitivity: 1.06 pg/ml
  • Range: 7.81 pg/ml - 500 pg/ml
  • Sample type: Cell culture extracts, Cell culture media, Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Serum
  • Detection method: Colorimetric
  • Assay type: Sandwich (quantitative)
  • Reacts with: Human

Overview

  • Product name

    Human Angiogenin ELISA Kit
    See all Angiogenin kits

  • Detection method

    Colorimetric

  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Serum 8 4.2%
    Inter-assay
    Sample n Mean SD CV%
    Serum 3 5.6%

  • Sample type

    Cell culture supernatant, Serum, Cell culture extracts, Cell culture media, Hep Plasma, EDTA Plasma, Cit plasma

  • Assay type

    Sandwich (quantitative)

  • Sensitivity

    1.06 pg/ml

  • Range

    7.81 pg/ml - 500 pg/ml

  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 110 108% - 113%
    Serum 103 99% - 106%
    Cell culture extracts 101 99% - 103%
    Cell culture media 104 100% - 107%
    Hep Plasma 103 96% - 109%
    EDTA Plasma 100 96% - 102%
    Cit plasma 101 97% - 105%

  • Assay time

    1h 30m

  • Assay duration

    One step assay

  • Species reactivity

    Reacts with: Human
    Does not react with: Cow

  • Product overview

    Human Angiogenin ELISA Kit (ab219629) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Angiogenin protein in cell culture extracts, cell culture supernatant, cit plasma, edta plasma, hep plasma, serum, and cell culture media. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Angiogenin with 1.06 pg/ml sensitivity.


    SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:


            - Single-wash protocol reduces assay time to 90 minutes or less
            - High sensitivity, specificity and reproducibility from superior antibodies
            - Fully validated in biological samples
            - 96-wells plate breakable into 12 x 8 wells strips


    A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

  • Notes

    Angiogenin is a member of the ribonuclease superfamily with approximately 35% amino acid sequence homology with pancreatic RNase. Angiogenin can act to inhibit protein synthesis by functioning as a cytotoxic tRNA-specific RNase.

    Cells expressing Angiogenin include vascular endothelial and smooth muscle cells, fibroblasts, colonic epithelium, peripheral blood lymphocytes, lung cell lines, and primary gastrointestinal adenocarcinomas. Basal levels of Angiogenin are also present in normal human serum and plasma. Angiogenin has been reported to serve as protection for motor neurons and it follows that Angiogenin is associated with neurological diseases, specifically, mutations to this gene are linked to Amyotrophic Lateral Sclerosis (ALS). Human and mouse Angiogenin share 74% homology in amino acid sequence.

    Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
    It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

Images

  • Other - Human Angiogenin ELISA Kit (ab219629)
    Other - Human Angiogenin ELISA Kit (ab219629)

    SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

     

  • Example of human Angiogenin standard curve in Sample Diluent NS.
    Example of human Angiogenin standard curve in Sample Diluent NS.

    Background-subtracted data values (mean +/- SD) are graphed.

  • Example of human Angiogenin standard curve in Sample Diluent 1X Cell Extraction Buffer PTR.
    Example of human Angiogenin standard curve in Sample Diluent 1X Cell Extraction Buffer PTR.

    Background-subtracted data values (mean +/- SD) are graphed.

  • Interpolated concentrations of native Angiogenin in human serum and plasma samples.
    Interpolated concentrations of native Angiogenin in human serum and plasma samples.

    The concentrations of Angiogenin were measured in duplicates, interpolated from the Angiogenin standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 1:1,000, plasma (citrate) 1:1,000, plasma (EDTA) 1:1,000, and plasma (heparin) 1:1,000. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Angiogenin concentration was determined to be 342.53 ng/mL in neat serum, 310.16 ng/mL in neat plasma (citrate), 249.90 ng/mL in neat plasma (EDTA), and 329.36 ng/mL in neat plasma (heparin).

  • Interpolated concentrations of spiked Angiogenin in RPMI culture media and native Angiogenin in HepG2 cell culture supernatant samples.
    Interpolated concentrations of spiked Angiogenin in RPMI culture media and native Angiogenin in HepG2 cell culture supernatant samples.

    The concentrations of Angiogenin were measured in duplicates, interpolated from the Angiogenin standard curves and corrected for sample dilution. Undiluted samples are as follows: RPMI culture media 50% and HepG2 supernatant 25%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Angiogenin concentration was determined to be 527.60 pg/mL in neat, spiked RPMI culture media. The mean Angiogenin concentration was determined to be 945.93 pg/mL in neat, native HepG2 supernatant.

  • Interpolated concentrations of native Angiogenin in HepG2 cell extract sample based on a 62.5 µg/mL extract load.
    Interpolated concentrations of native Angiogenin in HepG2 cell extract sample based on a 62.5 µg/mL extract load.

    The concentrations of Angiogenin were measured in duplicate and interpolated from the Angiogenin standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Angiogenin concentration was determined to be 304.03 pg/mL in HepG2 cell extract sample.

  • Serum from ten individual healthy human male donors was measured in duplicate.
    Serum from ten individual healthy human male donors was measured in duplicate.

    Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Angiogenin concentration was determined to be 495.29 ng/mL with a range of 214.57 – 824.72 ng/mL.

  • Sandwich ELISA - Human Angiogenin ELISA Kit (ab219629)
    Sandwich ELISA - Human Angiogenin ELISA Kit (ab219629)
    To learn more about the advantages of recombinant antibodies see here.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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