Human Angiogenin Matched Antibody Pair Kit (ab222263)
Key features and details
- Unlabeled capture antibody, biotin-labeled detection antibody and calibrated protein standard
- For economical ELISA and ELISA-based assay development
- Reacts with: Human
- Range: 7.8 pg/ml - 500 pg/ml
Overview
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Product name
Human Angiogenin Matched Antibody Pair Kit
See all Angiogenin kits -
Detection method
Colorimetric -
Assay type
ELISA set -
Sensitivity
3.5 pg/ml -
Range
7.8 pg/ml - 500 pg/ml -
Species reactivity
Reacts with: Human -
Product overview
Human Angiogenin Matched Antibody Pair Kits include a capture and a biotinylated detector antibody pair, along with a calibrated protein standard, suitable for sandwich ELISA. The Matched Antibody Pair Kit can be used to quantify native and recombinant human Angiogenin.
Optimization of the kit reagents to sample type, immunoassay format or instrumentation may be required. Guidelines for use of this kit in a standard 96-well microplate sandwich ELISA using HRP/TMB system of colorimetric detection is described in this assay procedure for the purposes of quantification.
Protocol information and tips on the use of the Matched Antibody Pair kits for sandwich ELISA can be found on our website. An accessory pack can be purchased which includes buffer reagents required to perform 10 x 96-well plate sandwich ELISAs (ab210905).
For additional information on the performance of the antibody pair used in this kit, please see our equivalent SimpleStep ELISA kit ab219629. Please note that while the antibody pair is the same provided in the corresponding SimpleStep ELISA Kit, due to differences in their formulation, this antibody pair cannot be used with the consumables provided with our SimpleStep ELISA Kits.
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Tested applications
Suitable for: ELISAmore details -
Platform
Reagents
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 10 x 96 tests 5 x 96 tests Human Angiogenin Capture Antibody 2 x 50µg 1 x 50µg Human Angiogenin Detector Antibody 2 x 12.5µg 1 x 12.5µg Human Angiogenin Lyophilized Protein 2 vials 1 vial -
Research areas
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Function
May function as a tRNA-specific ribonuclease that abolishes protein synthesis by specifically hydrolyzing cellular tRNAs. Binds to actin on the surface of endothelial cells; once bound, angiogenin is endocytosed and translocated to the nucleus. Angiogenin induces vascularization of normal and malignant tissues. Angiogenic activity is regulated by interaction with RNH1 in vivo. -
Tissue specificity
Expressed predominantly in the liver. Also detected in endothelial cells and spinal cord neurons. -
Involvement in disease
Defects in ANG are the cause of susceptibility to amyotrophic lateral sclerosis type 9 (ALS9) [MIM:611895]. ALS is a degenerative disorder of motor neurons in the cortex, brain stem and spinal cord. ALS is characterized by muscular weakness and atrophy. -
Sequence similarities
Belongs to the pancreatic ribonuclease family. -
Developmental stage
Low level expression in the developing fetus, increased in the neonate, and maximal in the adult. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- ALS9
- AMYOTROPHIC LATERAL SCLEROSIS
- ANG
see all -
Database links
- Entrez Gene: 283 Human
- Omim: 105850 Human
- SwissProt: P03950 Human
- Unigene: 283749 Human
Images
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Human Angiogenin standard curve
Standard calibration curve. Background subtracted values are graphed.
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Sandwich ELISA - Human Angiogenin Matched Antibody Pair Kit (ab222263)To learn more about the advantages of recombinant antibodies see here.
