Anti-Apolipoprotein A I antibody (ab64308)
Key features and details
- Rabbit polyclonal to Apolipoprotein A I
- Suitable for: Sandwich ELISA, WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Apolipoprotein A I antibody
See all Apolipoprotein A I primary antibodies -
Description
Rabbit polyclonal to Apolipoprotein A I -
Host species
Rabbit -
Tested applications
Suitable for: Sandwich ELISA, WB, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Baboon
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Immunogen
Synthetic peptide conjugated to KLH derived from within residues 200 to the C-terminus of Human Apolipoprotein A I.
Read Abcam's proprietary immunogen policy (Peptide available as ab66674.) -
Positive control
- Recombinant Human Apolipoprotein A I (ab50239) can be used as a positive control in WB. This antibody gave a positive signal in the following Human Tissue Lysates: Testis, Ovary, Lung, Thymus ICC/IF: HepG2 cells
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
Our Abpromise guarantee covers the use of ab64308 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application Abreviews Notes Sandwich ELISA Use a concentration of 0.5 µg/ml. For sandwich ELISA, use this antibody as Detection at 0.5µg/ml with ab20918 as Capture.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 27 kDa (predicted molecular weight: 31 kDa). ICC/IF Use a concentration of 5 µg/ml. Target
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Function
Participates in the reverse transport of cholesterol from tissues to the liver for excretion by promoting cholesterol efflux from tissues and by acting as a cofactor for the lecithin cholesterol acyltransferase (LCAT). As part of the SPAP complex, activates spermatozoa motility. -
Tissue specificity
Major protein of plasma HDL, also found in chylomicrons. Synthesized in the liver and small intestine. -
Involvement in disease
Defects in APOA1 are a cause of high density lipoprotein deficiency type 2 (HDLD2) [MIM:604091]; also known as familial hypoalphalipoproteinemia (FHA). Inheritance is autosomal dominant.
Defects in APOA1 are a cause of the low HDL levels observed in high density lipoprotein deficiency type 1 (HDLD1) [MIM:205400]; also known as analphalipoproteinemia or Tangier disease (TGD). HDLD1 is a recessive disorder characterized by the absence of plasma HDL, accumulation of cholesteryl esters, premature coronary artery disease, hepatosplenomegaly, recurrent peripheral neuropathy and progressive muscle wasting and weakness. In HDLD1 patients, ApoA-I fails to associate with HDL probably because of the faulty conversion of pro-ApoA-I molecules into mature chains, either due to a defect in the converting enzyme activity or a specific structural defect in Tangier ApoA-I.
Defects in APOA1 are the cause of amyloid polyneuropathy-nephropathy Iowa type (AMYLIOWA) [MIM:107680]; also known as amyloidosis van Allen type or familial amyloid polyneuropathy type III. AMYLIOWA is a hereditary generalized amyloidosis due to deposition of amyloid mainly constituted by apolipoprotein A1. The clinical picture is dominated by neuropathy in the early stages of the disease and nephropathy late in the course. Death is due in most cases to renal amyloidosis. Severe peptic ulcer disease can occurr in some and hearing loss is frequent. Cataracts is present in several, but vitreous opacities are not observed.
Defects in APOA1 are a cause of amyloidosis type 8 (AMYL8) [MIM:105200]; also known as systemic non-neuropathic amyloidosis or Ostertag-type amyloidosis. AMYL8 is a hereditary generalized amyloidosis due to deposition of apolipoprotein A1, fibrinogen and lysozyme amyloids. Viscera are particularly affected. There is no involvement of the nervous system. Clinical features include renal amyloidosis resulting in nephrotic syndrome, arterial hypertension, hepatosplenomegaly, cholestasis, petechial skin rash. -
Sequence similarities
Belongs to the apolipoprotein A1/A4/E family. -
Post-translational
modificationsPalmitoylated.
Phosphorylation sites are present in the extracelllular medium. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 335 Human
- Omim: 107680 Human
- SwissProt: P02647 Human
- Unigene: 93194 Human
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Alternative names
- Apo-AI antibody
- ApoA I antibody
- ApoA-I antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - Anti-Apolipoprotein A I antibody (ab64308)Lab
ab64308 staining Apolipoprotein A I in HepG2 cells. The cells were fixed with 4% paraformaldehyde (10 min), permeabilized with 0.1% PBS-Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab64308 at 5µg/ml and ab7291, Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control. Cells were then incubated with ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 488), pre-adsorbed at 1/1000 dilution (shown in green) and ab150120, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 594), pre-adsorbed at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).
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Western blot - Anti-Apolipoprotein A I antibody (ab64308)All lanes : Anti-Apolipoprotein A I antibody (ab64308) at 1 µg/ml
Lane 1 : Human testis tissue lysate - total protein (ab30257)
Lane 2 : Human ovary tissue lysate - total protein (ab30222)
Lane 3 : Lung (Human) Tissue Lysate - adult normal tissue
Lane 4 : Human thymus tissue lysate - total protein (ab30146)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 31 kDa
Observed band size: 27 kDa why is the actual band size different from the predicted? -
Sandwich ELISA - Anti-Apolipoprotein A I antibody (ab64308)Standard Curve for Apolipoprotein A I (Analyte: ab50239) dilution range 1pg/ml to 1ug/ml using Capture Antibody Mouse monoclonal [1409] to Apolipoprotein A I (ab20918) at 1ug/ml and Detector Antibody Rabbit polyclonal to Apolipoprotein A I (ab64308) at 0.5ug/ml
Protocols
References (6)
ab64308 has been referenced in 6 publications.
- Sai N et al. Involvement of Cholesterol Metabolic Pathways in Recovery from Noise-Induced Hearing Loss. Neural Plast 2020:6235948 (2020). PubMed: 32617095
- Noren Hooten N et al. Association of Extracellular Vesicle Protein Cargo with Race and Clinical Markers of Mortality. Sci Rep 9:17582 (2019). PubMed: 31772226
- Morishita H et al. A critical role of VMP1 in lipoprotein secretion. Elife 8:N/A (2019). PubMed: 31526472
- Lv P et al. Apolipoprotein C-III in the high-density lipoprotein proteome of cerebral lacunar infarction patients impairs its anti-inflammatory function. Int J Mol Med 41:61-68 (2018). PubMed: 29115584
- Domingo-Espín J et al. Site-specific glycations of apolipoprotein A-I lead to differentiated functional effects on lipid-binding and on glucose metabolism. Biochim Biophys Acta N/A:N/A (2018). PubMed: 29802959
- Holzer M et al. HDL structure and function is profoundly affected when stored frozen in the absence of cryoprotectants. J Lipid Res 58:2220-2228 (2017). PubMed: 28893842
Images
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Immunocytochemistry/ Immunofluorescence - Anti-Apolipoprotein A I antibody (ab64308) Lab
ab64308 staining Apolipoprotein A I in HepG2 cells. The cells were fixed with 4% paraformaldehyde (10 min), permeabilized with 0.1% PBS-Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab64308 at 5µg/ml and ab7291, Mouse monoclonal [DM1A] to alpha Tubulin - Loading Control. Cells were then incubated with ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 488), pre-adsorbed at 1/1000 dilution (shown in green) and ab150120, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 594), pre-adsorbed at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).
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Western blot - Anti-Apolipoprotein A I antibody (ab64308)All lanes : Anti-Apolipoprotein A I antibody (ab64308) at 1 µg/ml
Lane 1 : Human testis tissue lysate - total protein (ab30257)
Lane 2 : Human ovary tissue lysate - total protein (ab30222)
Lane 3 : Lung (Human) Tissue Lysate - adult normal tissue
Lane 4 : Human thymus tissue lysate - total protein (ab30146)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size: 31 kDa
Observed band size: 27 kDa why is the actual band size different from the predicted?
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Sandwich ELISA - Anti-Apolipoprotein A I antibody (ab64308)Standard Curve for Apolipoprotein A I (Analyte: ab50239) dilution range 1pg/ml to 1ug/ml using Capture Antibody Mouse monoclonal [1409] to Apolipoprotein A I (ab20918) at 1ug/ml and Detector Antibody Rabbit polyclonal to Apolipoprotein A I (ab64308) at 0.5ug/ml
