Anti-Apolipoprotein A I antibody [12C8] (ab17278)
![Anti-Apolipoprotein A I antibody [12C8] (ab17278)](https://www.abcam.com/ps/products/17/ab17278/Images/ab17278-26017-anti-apolipoprotein-a-i-antibody-12c8-western-blot.jpg "Anti-Apolipoprotein A I antibody [12C8] (ab17278)")
Key features and details
- Mouse monoclonal [12C8] to Apolipoprotein A I
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-Apolipoprotein A I antibody [12C8]
See all Apolipoprotein A I primary antibodies -
Description
Mouse monoclonal [12C8] to Apolipoprotein A I -
Host species
Mouse -
Specificity
No cross reactivity is seen with human apolipoprotein B. -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Full length native Apolipoprotein A1, isolated from human plasma.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. -
Storage buffer
pH: 7.40
Preservative: 0.097% Sodium azide
Constituents: 0.0268% PBS, 2.9% Sodium chloride -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
12C8 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Images
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Western blot - Anti-Apolipoprotein A I antibody [12C8] (ab17278)All lanes : Anti-Apolipoprotein A I antibody [12C8] (ab17278) at 5 µg/ml
Lane 1 : Human ovary tissue lysate - total protein (ab30222)
Lane 2 : Lung (Human) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
Lane 1 : Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Lane 2 : Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Predicted band size: 24 kDa
Observed band size: 25 kDa why is the actual band size different from the predicted?
Additional bands at: 260 kDa, 45 kDa, 55 kDa, 75 kDa. We are unsure as to the identity of these extra bands.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Apolipoprotein A I antibody [12C8] (ab17278)](https://www.abcam.com/ps/products/17/ab17278/Images/ab17278-26016-anti-apolipoprotein-a-i-antibody-12c8-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Apolipoprotein A I antibody [12C8] (ab17278)Ab17278 staining human normal skeletal muscle. Staining is localised to the cytoplasm.
Left panel: with primary antibody at 2 ug/ml. Right panel: isotype control.
Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffer EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS), then incubated with primary antibody for 20 minutes, and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
