Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Apolipoprotein A I antibody [EP1368Y] (ab52945)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP1368Y] to Apolipoprotein A I
  • Suitable for: Sandwich ELISA, WB, IP, Flow Cyt, IHC-P, ICC/IF, ELISA
  • Reacts with: Human, Recombinant fragment

Overview

  • Product name

    Anti-Apolipoprotein A I antibody [EP1368Y]
    See all Apolipoprotein A I primary antibodies

  • Description

    Rabbit monoclonal [EP1368Y] to Apolipoprotein A I

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ELISA
    Recombinant fragment
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Human
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human Apolipoprotein A I aa 1-100 (N terminal). The exact sequence is proprietary.

  • Positive control

    • WB: Human liver and fetal lysates IHC-P: Human liver Tissue ICC/IF: HepG2 cells Flow Cyt: HepG2 cells IP: HepG2 cell lysates, Human fetal liver tissue lysate

  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Images

  • Western blot - Anti-Apolipoprotein A I antibody [EP1368Y] (ab52945)
    Western blot - Anti-Apolipoprotein A I antibody [EP1368Y] (ab52945)
    Anti-Apolipoprotein A I antibody [EP1368Y] (ab52945) at 1/1000 dilution (Purified) + HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysates at 15 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 31 kDa
    Observed band size: 31 kDa

  • Western blot - Anti-Apolipoprotein A I antibody [EP1368Y] (ab52945)
    Western blot - Anti-Apolipoprotein A I antibody [EP1368Y] (ab52945)
    Anti-Apolipoprotein A I antibody [EP1368Y] (ab52945) at 1/1000 dilution (Purified) + Human liver lysates at 15 µg

    Secondary
    Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Predicted band size: 31 kDa
    Observed band size: 31 kDa

  • Western blot - Anti-Apolipoprotein A I antibody [EP1368Y] (ab52945)
    Western blot - Anti-Apolipoprotein A I antibody [EP1368Y] (ab52945)
    Anti-Apolipoprotein A I antibody [EP1368Y] (ab52945) at 1/20000 dilution ((unpurified)) + fetal liver lysate at 10 µg

    Secondary
    goat anti-rabbit HRP at 1/2000 dilution

    Predicted band size: 31 kDa
    Observed band size: 31 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Apolipoprotein A I antibody [EP1368Y] (ab52945)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Apolipoprotein A I antibody [EP1368Y] (ab52945)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human liver tissue sections labeling Apolipoprotein A I with purified ab52945 at 1/100 dilution (1.95 µg/ml). Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
  • Immunocytochemistry/ Immunofluorescence - Anti-Apolipoprotein A I antibody [EP1368Y] (ab52945)
    Immunocytochemistry/ Immunofluorescence - Anti-Apolipoprotein A I antibody [EP1368Y] (ab52945)

    Immunocytochemistry/ Immunofluorescence analysis of HepG2 (Human hepatocellular carcinoma epithelial cell) cells labeling Apolipoprotein A I with purified ab52945 at 1/250 dilution (0.8 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) at 1/200 (2.5 µg/ml) dilution. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

  • Flow Cytometry - Anti-Apolipoprotein A I antibody [EP1368Y] (ab52945)
    Flow Cytometry - Anti-Apolipoprotein A I antibody [EP1368Y] (ab52945)

    Flow Cytometry analysis of HepG2 (Human hepatocellular carcinoma epithelial cell) cells labeling Apolipoprotein A I with purified ab52945 at 1/20 dilution (10 µg/ml) (red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).

  • Immunoprecipitation - Anti-Apolipoprotein A I antibody [EP1368Y] (ab52945)
    Immunoprecipitation - Anti-Apolipoprotein A I antibody [EP1368Y] (ab52945)

    ab52945 (purified) at 1/20 dilution (1ug) immunoprecipitating Apolipoprotein A I in Human fetal liver lysates.
    Lane 1: Human fetal liver lysates 10ug
    Lane 2 (+): ab52945 & Human fetal liver lysates
    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab52945 in Human fetal liver lysates
    For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/1000 dilution.
    Blocking and diluting buffer: 5% NFDM/TBST.

  • ELISA - Anti-Apolipoprotein A I antibody [EP1368Y] (ab52945)
    ELISA - Anti-Apolipoprotein A I antibody [EP1368Y] (ab52945)

    ELISA analysis of Apolipoprotein A I recombinant protein at 1000 ng/mL with ab52945 at 1000~0ng/mL. An Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1/2500 dilution was used as the secondary antibody.

  • ELISA - Anti-Apolipoprotein A I antibody [EP1368Y] (ab52945)
    ELISA - Anti-Apolipoprotein A I antibody [EP1368Y] (ab52945)

    Direct ELISA antigen dose-response curve using purified ab52945 at 0-1000ng/mL. Antigen (apo AI peptide) concentration of 1000ng/mL. An Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) (1/2500) was used as the secondary antibody.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Apolipoprotein A I antibody [EP1368Y] (ab52945)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Apolipoprotein A I antibody [EP1368Y] (ab52945)

    ab52945 (unpurified) at 1/100 dilution staining Apolipoprotein A I in human liver by Immunohistochemistry, Paraffin embedded tissue.

  • Immunocytochemistry/ Immunofluorescence - Anti-Apolipoprotein A I antibody [EP1368Y] (ab52945)
    Immunocytochemistry/ Immunofluorescence - Anti-Apolipoprotein A I antibody [EP1368Y] (ab52945)

    ab52945 (unpurified) at 1/100 dilution staining Apolipoprotein A I in HEPG2 cells by Immunofluorescence.

  • Anti-Apolipoprotein A I antibody [EP1368Y] (ab52945)
    Anti-Apolipoprotein A I antibody [EP1368Y] (ab52945)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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