Antibodies, Proteins, Kits and Reagents for Life Science

355 142 ₸ Product size

100 µl 355 142 ₸ 10 µl 140 716 ₸

Order now and get it on Tuesday March 02, 2021

Anti-Apolipoprotein A I antibody [EP1368Y] (ab52945)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EP1368Y] to Apolipoprotein A I
Suitable for: Sandwich ELISA, WB, IP, Flow Cyt, IHC-P, ICC/IF, ELISA
Reacts with: Human, Recombinant fragment

Product name

Anti-Apolipoprotein A I antibody [EP1368Y]
See all Apolipoprotein A I primary antibodies
Description

Rabbit monoclonal [EP1368Y] to Apolipoprotein A I
Host species

Rabbit

Tested Applications & Species

Application	Species
ELISA	Recombinant fragment
Flow Cyt	Human
ICC/IF	Human
IHC-P	Human
IP	Human
WB	Human

See all applications and species data

Immunogen

Synthetic peptide within Human Apolipoprotein A I aa 1-100 (N terminal). The exact sequence is proprietary.
Positive control
- WB: Human liver and fetal lysates IHC-P: Human liver Tissue ICC/IF: HepG2 cells Flow Cyt: HepG2 cells IP: HepG2 cell lysates, Human fetal liver tissue lysate
General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Stable for 12 months at -20°C.
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EP1368Y
Isotype

IgG
Research areas

Western blot - Anti-Apolipoprotein A I antibody [EP1368Y] (ab52945)

Anti-Apolipoprotein A I antibody [EP1368Y] (ab52945) at 1/1000 dilution (Purified) + HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysates at 15 µg

Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 31 kDa
Observed band size: 31 kDa
Western blot - Anti-Apolipoprotein A I antibody [EP1368Y] (ab52945)

Anti-Apolipoprotein A I antibody [EP1368Y] (ab52945) at 1/1000 dilution (Purified) + Human liver lysates at 15 µg

Secondary
Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Predicted band size: 31 kDa
Observed band size: 31 kDa
Western blot - Anti-Apolipoprotein A I antibody [EP1368Y] (ab52945)

Anti-Apolipoprotein A I antibody [EP1368Y] (ab52945) at 1/20000 dilution ((unpurified)) + fetal liver lysate at 10 µg

Secondary
goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 31 kDa
Observed band size: 31 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Apolipoprotein A I antibody [EP1368Y] (ab52945)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human liver tissue sections labeling Apolipoprotein A I with purified ab52945 at 1/100 dilution (1.95 µg/ml). Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
Immunocytochemistry/ Immunofluorescence - Anti-Apolipoprotein A I antibody [EP1368Y] (ab52945)

Immunocytochemistry/ Immunofluorescence analysis of HepG2 (Human hepatocellular carcinoma epithelial cell) cells labeling Apolipoprotein A I with purified ab52945 at 1/250 dilution (0.8 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) at 1/200 (2.5 µg/ml) dilution. Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) was used as the secondary antibody at 1/1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
Flow Cytometry - Anti-Apolipoprotein A I antibody [EP1368Y] (ab52945)

Flow Cytometry analysis of HepG2 (Human hepatocellular carcinoma epithelial cell) cells labeling Apolipoprotein A I with purified ab52945 at 1/20 dilution (10 µg/ml) (red). Cells were fixed with 4% Paraformaldehyde and permeabilised with 90% Methanol. A Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) secondary antibody was used at 1/2000. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
Immunoprecipitation - Anti-Apolipoprotein A I antibody [EP1368Y] (ab52945)

ab52945 (purified) at 1/20 dilution (1ug) immunoprecipitating Apolipoprotein A I in Human fetal liver lysates.
Lane 1: Human fetal liver lysates 10ug
Lane 2 (+): ab52945 & Human fetal liver lysates
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab52945 in Human fetal liver lysates
For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/1000 dilution.
Blocking and diluting buffer: 5% NFDM/TBST.
ELISA - Anti-Apolipoprotein A I antibody [EP1368Y] (ab52945)

ELISA analysis of Apolipoprotein A I recombinant protein at 1000 ng/mL with ab52945 at 1000~0ng/mL. An Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1/2500 dilution was used as the secondary antibody.
ELISA - Anti-Apolipoprotein A I antibody [EP1368Y] (ab52945)

Direct ELISA antigen dose-response curve using purified ab52945 at 0-1000ng/mL. Antigen (apo AI peptide) concentration of 1000ng/mL. An Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) (1/2500) was used as the secondary antibody.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Apolipoprotein A I antibody [EP1368Y] (ab52945)

ab52945 (unpurified) at 1/100 dilution staining Apolipoprotein A I in human liver by Immunohistochemistry, Paraffin embedded tissue.
Immunocytochemistry/ Immunofluorescence - Anti-Apolipoprotein A I antibody [EP1368Y] (ab52945)

ab52945 (unpurified) at 1/100 dilution staining Apolipoprotein A I in HEPG2 cells by Immunofluorescence.
Anti-Apolipoprotein A I antibody [EP1368Y] (ab52945)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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