Anti-DCTN6/WS3 antibody (ab230014)
Key features and details
- Rabbit polyclonal to DCTN6/WS3
- Suitable for: WB, IP, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-DCTN6/WS3 antibody
See all DCTN6/WS3 primary antibodies -
Description
Rabbit polyclonal to DCTN6/WS3 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IP, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Cow, Orangutan
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Immunogen
Recombinant full length protein corresponding to Human DCTN6/WS3 aa 1 to the C-terminus.
Database link: O00399 -
Positive control
- WB: Mouse lung, stomach, thymus and gonadal lysates; A549 and U-251 MG whole cell lysates; Rat brain lysate. IHC-P: Human gastric cancer and kidney tissues. IP: A549 whole cell lysate.
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General notes
This product was previously labelled as DCTN6
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-DCTN6/WS3 antibody (ab230014)All lanes : Anti-DCTN6/WS3 antibody (ab230014) at 1/1000 dilution
Lane 1 : Mouse lung lysate
Lane 2 : Mouse gonadal lysate
Lane 3 : A549 (human lung carcinoma cell line) whole cell lysate
Lane 4 : U-251 MG (human brain glioma cell line) whole cell lysate
Lane 5 : Mouse thymus lysate
Lane 6 : Rat brain lysate
Lane 7 : Mouse stomach lysate
Secondary
All lanes : Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 21 kDa
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DCTN6/WS3 antibody (ab230014)Paraffin-embedded human kidney tissue staned for DCTN6/WS3 using ab230014 at 1/100 dilution in immunohistochemical analysis.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DCTN6/WS3 antibody (ab230014)Paraffin-embedded human gastric cancer tissue stained for DCTN6/WS3 using ab230014 at 1/100 dilution in immunohistochemical analysis.
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Immunoprecipitation - Anti-DCTN6/WS3 antibody (ab230014)DCTN6/WS3 was immunoprecipitated from 500 µg A549 (human lung carcinoma cell line) whole cell lysate with 1 µg of ab230014.
Lane 1: 1 µg rabbit monoclonal IgG instead of ab230014 in A549 whole cell lysate.
Lane 2: ab230014 IP in A549 whole cell lysate.
Lane 3: A549 whole cell lysate 20 µg (input).
For western blotting, an HRP-conjugated light chain specific antibody was used as the Secondary antibody at 1/50000 dilution.
