Anti-p53 (phospho S376) antibody [EPR17730] (ab183547)
![Anti-p53 (phospho S376) antibody [EPR17730] (ab183547)](https://www.abcam.com/ps/products/183/ab183547/Images/ab183547-235949-anti-p53-phospho-s376-antibody-epr17730-western-blot.jpg "Anti-p53 (phospho S376) antibody [EPR17730] (ab183547)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR17730] to p53 (phospho S376)
- Suitable for: Dot blot, WB
- Reacts with: Human
Overview
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Product name
Anti-p53 (phospho S376) antibody [EPR17730]
See all p53 primary antibodies -
Description
Rabbit monoclonal [EPR17730] to p53 (phospho S376) -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Whole cell lysate of HEK-293 cells treated with 200nM Calyculin A and 1uM Okadaic Acid for 60 minutes.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR17730 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-p53 (phospho S376) antibody [EPR17730] (ab183547)All lanes : Anti-p53 (phospho S376) antibody [EPR17730] (ab183547) at 1/1000 dilution
Lane 1 : Whole cell lysate of HEK-293 (Human epithelial cells from embryonic kidney) treated with 200nM Calyculin A and 1uM Okadaic Acid for 60 minutes.
Lane 2 : Untreated whole cell lysate of HEK-293
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution
Predicted band size: 43 kDa
Observed band size: 53 kDa why is the actual band size different from the predicted?Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure time of 15 seconds.
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![Dot Blot - Anti-p53 (phospho S376) antibody [EPR17730] (ab183547)](https://www.abcam.com/ps/products/183/ab183547/Images/ab183547-235948-anti-p53-phospho-s376-antibody-epr17730-dot-blot.jpg)
Dot Blot - Anti-p53 (phospho S376) antibody [EPR17730] (ab183547)Dot blot analysis of p53 (phospho S376) using ab183547 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated secondary antibody at 1/1000 dilution.
Lane 1: Phospho peptide
Lane 2: Non-phospho peptide
Blocking/Dilution buffer: 5% NFDM/TBST.
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![Anti-p53 (phospho S376) antibody [EPR17730] (ab183547)](https://www.abcam.com/ps/products/183/ab183547/Images/ab183547-3-benefits-of-recombinant-antibodies.png)
Anti-p53 (phospho S376) antibody [EPR17730] (ab183547)
