Antibodies, Proteins, Kits and Reagents for Life Science

311 587 ₸ Product size

100 µl 311 587 ₸

Order now and get it on Tuesday March 02, 2021

Anti-Activin A Receptor Type IB/ALK-4 antibody [EPR4815] (ab109300)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR4815] to Activin A Receptor Type IB/ALK-4
Suitable for: WB, IHC-P, ICC/IF
Reacts with: Mouse, Rat, Human

Product name

Anti-Activin A Receptor Type IB/ALK-4 antibody [EPR4815]
See all Activin A Receptor Type IB/ALK-4 primary antibodies
Description

Rabbit monoclonal [EPR4815] to Activin A Receptor Type IB/ALK-4
Host species

Rabbit

Tested Applications & Species

Application	Species
ICC/IF	Human
IHC-P	Human
WB	Mouse Human

See all applications and species data

Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- Human, mouse and rat brain tissue lysate; Human fetal kidney and liver tissue lysate; Human cervical carcinoma; Human kidney tissue; SH-SY5Y and U87-MG cell lysate.
General notes
Previously labelled as Activin A Receptor Type IB.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Stable for 12 months at -20°C.
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 59% PBS, 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR4815
Isotype

IgG
Research areas

Western blot - Anti-Activin A Receptor Type IB/ALK-4 antibody [EPR4815] (ab109300)

Anti-Activin A Receptor Type IB/ALK-4 antibody [EPR4815] (ab109300) at 1/1000 dilution (purified) + Human fetal kidney lysate at 15 µg

Secondary
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution

Predicted band size: 57 kDa
Observed band size: 52 kDa
why is the actual band size different from the predicted?

Blocking/Diluting buffer 5% NFDM/TBST
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Activin A Receptor Type IB/ALK-4 antibody [EPR4815] (ab109300)

Immunohistochemical analysis of paraffin-embedded Human cervical carcinoma sections labelling Activin A Receptor Type IB/ALK-4 with purified ab109300 at dilution of 1/600. The secondary antibody used was ab97051; a goat anti-rabbit IgG H&L (HRP) at dilution of 1/500. The sample was counterstained with hematoxylin. Antigen retrieval was heat mediated using EDTA Buffer; pH 9.0. PBS was used instead of the primary antibody as the negative control and is shown in the inset.
Immunocytochemistry/ Immunofluorescence - Anti-Activin A Receptor Type IB/ALK-4 antibody [EPR4815] (ab109300)

Immunocytochemistry/Immunofluorescence analysis of SH-SY5Y (Human neuroblastoma cell line from bone marrow) cells labelling Activin A Receptor Type IB/ALK-4 with purified ab109300 at 1/250 (5.0 μg/mL). Cells were fixed with 4% Paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, Alexa Fluor^® 488-conjugated goat anti-rabbit IgG (1/1000, 2 μg/mL) was used as the secondary antibody. Cells were counter-stained with ab195889 Anti-Alpha Tubulin antibody [DM1A] (1/200, 2.5 μg/mL) - Microtubule Marker (Alexa Fluor^® 594). DAPI (blue) was used as a nuclear counterstain.

Secondary Only Control: PBS was used instead of the primary antibody as the negative control.
Western blot - Anti-Activin A Receptor Type IB/ALK-4 antibody [EPR4815] (ab109300)

All lanes : Anti-Activin A Receptor Type IB/ALK-4 antibody [EPR4815] (ab109300) at 1/1000 dilution (purified)

Lane 1 : Mouse brain tissue lysate
Lane 2 : Rat brain tissue lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 57 kDa
Observed band size: 52 kDa why is the actual band size different from the predicted?

Blocking/Diluting buffer 5% NFDM/TBST
Western blot - Anti-Activin A Receptor Type IB/ALK-4 antibody [EPR4815] (ab109300)

All lanes : Anti-Activin A Receptor Type IB/ALK-4 antibody [EPR4815] (ab109300) at 1/1000 dilution (unpurified)

Lane 1 : Human brain tissue lysate
Lane 2 : Human fetal kidney
Lane 3 : SH-SY5Y cell lysate
Lane 4 : U87-MG cell lysate
Lane 5 : Human fetal liver

Lysates/proteins at 10 µg per lane.

Predicted band size: 57 kDa
Observed band size: 52 kDa why is the actual band size different from the predicted?
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Activin A Receptor Type IB/ALK-4 antibody [EPR4815] (ab109300)

Immunohistochemical analysis of human kidney tissue sections labelling Activin A Receptor Type IB/ALK-4 with unpurified ab109300 at 1/500 dilution.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Immunocytochemistry/ Immunofluorescence - Anti-Activin A Receptor Type IB/ALK-4 antibody [EPR4815] (ab109300)

Immunocytochemistry/ Immunofluorescence analysis of 2% paraformaldehyde fixed (20 mins) SH-SY5Y cells labelling Activin A Receptor Type IB/ALK-4 with unpurified ab109300 at a dilution of 1/100. Alexa Fluor488 goat anti-rabbit IgG was used as the secondary antibody.
Anti-Activin A Receptor Type IB/ALK-4 antibody [EPR4815] (ab109300)

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