Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Cortactin antibody [4F11] - BSA and Azide free (ab269977)

Key features and details

  • Mouse monoclonal [4F11] to Cortactin - BSA and Azide free
  • Suitable for: ICC/IF, IHC-P, WB
  • Knockout validated
  • Reacts with: Mouse, Human
  • Isotype: IgG1

Overview

  • Product name

    Anti-Cortactin antibody [4F11] - BSA and Azide free
    See all Cortactin primary antibodies

  • Description

    Mouse monoclonal [4F11] to Cortactin - BSA and Azide free

  • Host species

    Mouse

  • Tested applications

    Suitable for: ICC/IF, IHC-P, WBmore details

  • Species reactivity

    Reacts with: Mouse, Human

  • Immunogen

    Full length native protein (purified). This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HAP1-Wild Type. NIH3T3 and A431 whole cell lysate. ICC/IF: HAP1-Wild Type. IHC-P: FFPE Human Breast Cancer tissue sections.

  • General notes

    This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.

    ab269977 is the carrier-free version of ab33333. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Images

  • Western blot - Anti-Cortactin antibody [4F11] - BSA and Azide free (ab269977)
    Western blot - Anti-Cortactin antibody [4F11] - BSA and Azide free (ab269977)
    All lanes : Anti-Cortactin antibody [4F11] (ab33333) at 1 µg/ml

    Lane 1 : HAP1 whole cell lysate
    Lane 2 : HAP1 CTTN whole cell lysate
    Lane 3 : NIH3T3 whole cell lysate
    Lane 4 : A431 whole cell lysate
    Lane 5 : PC12 whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Performed under reducing conditions.


    This data was developed using the same antibody clone in a different buffer formulation containing PBS and sodium azide (ab33333).

    ab33333 was shown to specifically react with CTTN (Cortactin) in wild type HAP1 cells. No band was observed when CTTN (Cortactin) knockout samples were used. Wild-type and CTTN (Cortactin) knockout samples were subjected to SDS-PAGE. ab33333 and ab181602 (Rabbit anti GAPDH) were incubated overnight at 4°C at a 1ug/ml concentration and 1/10000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

  • Immunocytochemistry/ Immunofluorescence - Anti-Cortactin antibody [4F11] - BSA and Azide free (ab269977)
    Immunocytochemistry/ Immunofluorescence - Anti-Cortactin antibody [4F11] - BSA and Azide free (ab269977)

    This data was developed using the same antibody clone in a different buffer formulation containing PBS and sodium azide (ab33333).

    ab33333 staining Cortactin in wild-type HAP1 cells (top panel) and CTTN knockout HAP1 cells (bottom panel). The cells were fixed with 100% methanol (5min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab33333 at 1μg/ml and ab7291 at 1μg/ml overnight at +4°C, followed by a further incubation at room temperature for 1h with a goat secondary antibody to Rabbit IgG (Alexa Fluor® 488) (ab150081) at 2 μg/ml (shown in green) and goat secondary antibody to Mouse IgG (Alexa Fluor® 594) (ab150120) at 2 μg/ml (shown in pseudo colour red). Nuclear DNA was labeled in blue with DAPI.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cortactin antibody [4F11] - BSA and Azide free (ab269977)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Cortactin antibody [4F11] - BSA and Azide free (ab269977)

    This data was developed using the same antibody clone in a different buffer formulation containing PBS and sodium azide (ab33333).

    IHC image of Cortactin staining in a section of formalin-fixed paraffin-embedded normal human breast carcinoma* performed on a Leica BONDTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab33333, 0.05ug/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset secondary-only control image is taken from an identical assay without primary antibody.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

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