Antibodies, Proteins, Kits and Reagents for Life Science

324 988 ₸ Product size

100 µl 324 988 ₸

Order now and get it on Tuesday March 09, 2021

Anti-Cortactin antibody [EPR1923Y] (ab68438)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR1923Y] to Cortactin
Suitable for: WB, Flow Cyt
Knockout validated
Reacts with: Mouse, Rat, Human

Product name

Anti-Cortactin antibody [EPR1923Y]
See all Cortactin primary antibodies
Description

Rabbit monoclonal [EPR1923Y] to Cortactin
Host species

Rabbit
Tested Applications & Species

Application Species
Flow Cyt
Human

WB
Rat

Human

See all applications and species data
Immunogen

Synthetic peptide within Human Cortactin aa 500-600 (C terminal). The exact sequence is proprietary.
Positive control
- C6, MCF-7, Hela and A431 cell lysate and permeabilized MCF-7 cells.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Application	Species
Flow Cyt	Human
WB	Rat Human

Form

Liquid
Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer

pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR1923Y
Isotype

IgG
Research areas

Western blot - Anti-Cortactin antibody [EPR1923Y] (ab68438)

Lane 1: Wild-type HAP1 cell lysate (40 µg)
Lane 2: Cortactin knockout HAP1 cell lysate (40 µg)
Lane 3: NIH3T3 cell lysate (20 µg)

Lanes 1 - 4: Merged signal (red and green). Green - ab68438 observed at 70 kDa. Red - loading control, ab8245, observed at 37 kDa.

ab68438 was shown to specifically recognize Cortactin in wild-type HAP1 cells along with additional cross-reactive bands. No band was observed when Cortactin knockout samples were examined. Wild-type and Cortactin knockout samples were subjected to SDS-PAGE. Ab68438 and ab8245 (loading control to GAPDH) were diluted at 1/1000 and 1/10,000 dilution respectively and incubated overnight at 4C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed ab216776 secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.
Western blot - Anti-Cortactin antibody [EPR1923Y] (ab68438)

All lanes : Anti-Cortactin antibody [EPR1923Y] (ab68438) at 1/5000 dilution

Lane 1 : C6 cell lysate
Lane 2 : MCF-7 cell lysate
Lane 3 : Hela cell lysate
Lane 4 : A431 cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 80 kDa
Observed band size: 80 kDa
Additional bands at: 75 kDa. We are unsure as to the identity of these extra bands.
Flow Cytometry - Anti-Cortactin antibody [EPR1923Y] (ab68438)

Flow cytometric analysis of permeabilized MCF-7 cells using ab68438 1:30 dilution (pink) or a rabbit IgG (negative) (green).
Anti-Cortactin antibody [EPR1923Y] (ab68438)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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