Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Cortactin antibody [EPR1923Y] - BSA and Azide free (ab247383)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR1923Y] to Cortactin - BSA and Azide free
  • Suitable for: WB, Flow Cyt
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Cortactin antibody [EPR1923Y] - BSA and Azide free
    See all Cortactin primary antibodies

  • Description

    Rabbit monoclonal [EPR1923Y] to Cortactin - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, Flow Cytmore details
    Unsuitable for: IHC-P or IP

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab247383 is the carrier-free version of ab68438. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab247383 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-Cortactin antibody [EPR1923Y] - BSA and Azide free (ab247383)
    Western blot - Anti-Cortactin antibody [EPR1923Y] - BSA and Azide free (ab247383)

    This data was developed using ab68438, the same antibody clone in a different buffer formulation.

    Lane 1: Wild-type HAP1 cell lysate (40 µg)

    Lane 2: Cortactin knockout HAP1 cell lysate (40 µg)

    Lane 3: NIH3T3 cell lysate (20 µg)

    Lanes 1 - 4: Merged signal (red and green). Green - ab68438 observed at 70 kDa. Red - loading control, ab8245, observed at 37 kDa.

    ab68438 was shown to specifically recognize Cortactin in wild-type HAP1 cells along with additional cross-reactive bands. No band was observed when Cortactin knockout samples were examined. Wild-type and Cortactin knockout samples were subjected to SDS-PAGE. ab68438 and ab8245 (loading control to GAPDH) were diluted at 1/1000 and 1/10,000 dilution respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.

  • Western blot - Anti-Cortactin antibody [EPR1923Y] - BSA and Azide free (ab247383)
    Western blot - Anti-Cortactin antibody [EPR1923Y] - BSA and Azide free (ab247383)
    All lanes : Anti-Cortactin antibody [EPR1923Y] (ab68438) at 1/5000 dilution

    Lane 1 : C6 cell lysate
    Lane 2 : MCF-7 cell lysate
    Lane 3 : HeLa cell lysate
    Lane 4 : A431 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 80 kDa
    Observed band size: 80 kDa



    This data was developed using ab68438, the same antibody clone in a different buffer formulation.

  • Flow Cytometry - Anti-Cortactin antibody [EPR1923Y] - BSA and Azide free (ab247383)
    Flow Cytometry - Anti-Cortactin antibody [EPR1923Y] - BSA and Azide free (ab247383)
    This data was developed using ab68438, the same antibody clone in a different buffer formulation.Flow cytometric analysis of permeabilized MCF-7 cells using ab68438 1:30 dilution (pink) or a rabbit IgG (negative) (green).
  • Anti-Cortactin antibody [EPR1923Y] - BSA and Azide free (ab247383)
    Anti-Cortactin antibody [EPR1923Y] - BSA and Azide free (ab247383)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Alternative products to Anti-Cortactin antibody [EPR1923Y] - BSA and Azide free (ab247383)

Clear all

Recently viewed products

© 2021 Astana Biomed Group LLP. All rights reserved.