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Anti-RhoA antibody [1B12] (ab54835)

Key features and details

  • Mouse monoclonal [1B12] to RhoA
  • Suitable for: WB, IHC-P, Flow Cyt, ICC/IF
  • Reacts with: Human
  • Isotype: IgG1

Overview

  • Product name

    Anti-RhoA antibody [1B12]
    See all RhoA primary antibodies

  • Description

    Mouse monoclonal [1B12] to RhoA

  • Host species

    Mouse

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant full length protein corresponding to Human RhoA aa 1-193.
    Database link: P61586

  • Positive control

    • WB: 293T, HL-60, lysate ICC/IF: HeLa cells. Flow cyt: HeLa cells. IHC-P: Human lymphoma tissue.

  • General notes

    This product was changed from ascites to tissue culture supernatant on 15 May 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

Properties

Images

  • Western blot - Anti-RhoA antibody [1B12] (ab54835)
    Western blot - Anti-RhoA antibody [1B12] (ab54835)

    RhoA antibody (ab54835) at 1ug/lane + HL-60 cell lysate (ab7914) at 25ug/lane.

    This image was generated using the ascites version of the product.

  • Western blot - Anti-RhoA antibody [1B12] (ab54835)
    Western blot - Anti-RhoA antibody [1B12] (ab54835)
    All lanes : Anti-RhoA antibody [1B12] (ab54835) at 1 µg

    Lane 1 : RHOA transfected 293T lysate
    Lane 2 : Non-transfected lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    All lanes : Goat anti-mouse IgG

    Predicted band size: 22 kDa



    This image was generated using the ascites version of the product.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RhoA antibody [1B12] (ab54835)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RhoA antibody [1B12] (ab54835)

    Paraffin embedded human lymphoma tissue stained for RhoA using ab54835 at 5 µg/ml in immunohistochemical analysis.

  • Immunocytochemistry/ Immunofluorescence - Anti-RhoA antibody [1B12] (ab54835)
    Immunocytochemistry/ Immunofluorescence - Anti-RhoA antibody [1B12] (ab54835)

    ICC/IF image of ab54835 stained HeLa cells (ab150035). The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab54835, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    This image was generated using the ascites version of the product.

  • Flow Cytometry - Anti-RhoA antibody [1B12] (ab54835)
    Flow Cytometry - Anti-RhoA antibody [1B12] (ab54835)

    Overlay histogram showing HeLa cells (ab150035) stained with ab54835 (red line). The cells were fixed with methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab54835, 1µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a decreased signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.
    This image was generated using the ascites version of the product.

  • Immunocytochemistry/ Immunofluorescence - Anti-RhoA antibody [1B12] (ab54835)
    Immunocytochemistry/ Immunofluorescence - Anti-RhoA antibody [1B12] (ab54835)

    ab54835 at 10 ug/ml staining RhoA in human Hela cells (ab150035) by Immunocytochemistry / Immunofluorescence.

    This image was generated using the ascites version of the product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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