Anti-RhoA (phospho S188) antibody (ab41435)
Key features and details
- Rabbit polyclonal to RhoA (phospho S188)
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-RhoA (phospho S188) antibody
See all RhoA primary antibodies -
Description
Rabbit polyclonal to RhoA (phospho S188) -
Host species
Rabbit -
Specificity
Phospho-Ser188-RhoA specific. RhoA immunogen sequence not found in RhoB or RhoC proteins. -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide phosphorylated at Ser188, corresponding to amino acid residues around serine 188 of human RhoA, conjugated to KLH.
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General notes
Do not aliquot.Post-translational regulation of Rho activity has been shown specifically for RhoA. This Rho protein is phosphorylated in vitro on serine 188 by cAMP- and cGMP-dependent kinases (PKA and PKG). Ser188 phosphorylation enhances RhoGDI binding and inhibits RhoA-mediated stress fiber formation. Thus, Ser188 is an important site for negative regulation of RhoA activity.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at 4°C (up to 6 months). Store at -20°C. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituents: PBS, 50% Glycerol, 0.1% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Primary antibody notes
Post-translational regulation of Rho activity has been shown specifically for RhoA. This Rho protein is phosphorylated in vitro on serine 188 by cAMP- and cGMP-dependent kinases (PKA and PKG). Ser188 phosphorylation enhances RhoGDI binding and inhibits RhoA-mediated stress fiber formation. Thus, Ser188 is an important site for negative regulation of RhoA activity. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-RhoA (phospho S188) antibody (ab41435)Lanes 1-2 : anti-Rho
Lanes 3-6 : Anti-RhoA (phospho S188) antibody (ab41435) at 1/1000 dilution
Lane 1 : recombinant human RhoA GST fusion protein, unphosphorylated
Lane 2 : recombinant human RhoA GST fusion protein, phosphorylated with PKA
Lane 3 : recombinant human RhoA GST fusion protein, unphosphorylated
Lane 4 : recombinant human RhoA GST fusion protein, phosphorylated with PKA
Lane 5 : recombinant human RhoA GST fusion protein, phosphorylated with PKA
with RhoA (phospho S188) peptide
Lane 6 : recombinant human RhoA GST fusion protein, phosphorylated with PKA with non-specific phosphoserine peptide
Predicted band size: 22 kDa
Observed band size: 49 kDa why is the actual band size different from the predicted?
Membrane was incubated with diluted antibody in 5% non-fat milk, PBS, 0.04% Tween20 for 1 hour at room temperature.A fusion protein was used in this western blot; hence the difference in molecular weight compared to predicted molecular weight.
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Western blot - Anti-RhoA (phospho S188) antibody (ab41435) This image is courtesy of an anonymous abreview.All lanes : Anti-RhoA (phospho S188) antibody (ab41435) at 1/1000 dilution
Lane 1 : MCF7
Lane 2 : human liver nuclear tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Polyclonal goat anti-rabbit HRP conjugate at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 22 kDa
Observed band size: 22 kDa
Exposure time: 25 secondsBlocking: 5% milk for 1 hour at 23°C
