Anti-Progesterone Receptor (phospho S294) antibody (ab61785)
Key features and details
- Rabbit polyclonal to Progesterone Receptor (phospho S294)
- Suitable for: ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-Progesterone Receptor (phospho S294) antibody
See all Progesterone Receptor primary antibodies -
Description
Rabbit polyclonal to Progesterone Receptor (phospho S294) -
Host species
Rabbit -
Specificity
Detects endogenous levels of Progesterone receptor only when phosphorylated at serine 294. -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanWB Human
-
Immunogen
Synthetic phosphopeptide derived from human Progesterone Receptor around the phosphorylation site of serine 294 (GRSPPL)
-
Positive control
- Extracts from Jurkat cells, treated with Etoposide (WB) Hela cells (IF)
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Without Mg2+ and Ca2+ -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Affinity purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
-
Immunocytochemistry/ Immunofluorescence - Anti-Progesterone Receptor (phospho S294) antibody (ab61785)Immunofluorescence analysis of HeLa cells, using ab61784 antibody at 1/500 dilution in the presence and abscence of immunizing peptide.
-
Western blot - Anti-Progesterone Receptor (phospho S294) antibody (ab61785)All lanes : Anti-Progesterone Receptor (phospho S294) antibody (ab61785) at 1/500 dilution
Lane 1 : extracts from Jurkat cells, treated
with Etoposide (25uM, 60mins) with no immunizing peptide
Lane 2 : extracts from Jurkat cells, treated
with Etoposide (25uM, 60mins) with immnunizing peptide
Predicted band size: 99 kDa
