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Epigenetics and Nuclear Signaling Transcription Domain Families Zinc Finger

Anti-Progesterone Receptor (phospho S190) antibody [EP1516Y] (ab75857)

Price and availability

241 228 ₸

Availability

Order now and get it on Tuesday March 09, 2021

Anti-Progesterone Receptor (phospho S190) antibody [EP1516Y] (ab75857)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP1516Y] to Progesterone Receptor (phospho S190)
  • Suitable for: WB, IHC-P
  • Reacts with: Human

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Overview

  • Product name

    Anti-Progesterone Receptor (phospho S190) antibody [EP1516Y]
    See all Progesterone Receptor primary antibodies
  • Description

    Rabbit monoclonal [EP1516Y] to Progesterone Receptor (phospho S190)
  • Host species

    Rabbit
  • Specificity

    ab75857 detects Progesterone Receptor phophorylated at serine 190.
  • Tested Applications & Species

    Application Species
    IHC-P
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    A phospho specific peptide corresponding to residues surrounding serine 190 of human Progesterone Receptor

  • Positive control

    • T47D cell lysates, untreated or treated with progesterone; human breast carcinoma tissue.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EP1516Y
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Transcription
    • Domain Families
    • Zinc Finger
    • Signal Transduction
    • Signaling Pathway
    • Nuclear Signaling
    • Nuclear Hormone Receptors
    • Progesterone
    • Neuroscience
    • Endocrine system
    • Gonadotrophic axis
    • Epigenetics and Nuclear Signaling
    • Nuclear Signaling Pathways
    • Nuclear Receptors
    • Progesterone
    • Cancer
    • Signal transduction
    • Nuclear signaling
    • Nuclear hormone receptors
    • Progesterone
    • Cancer
    • Tumor biomarkers
    • Receptors

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Progesterone Receptor (phospho S190) antibody [EP1516Y] (ab75857)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Progesterone Receptor (phospho S190) antibody [EP1516Y] (ab75857)

    ab75857 at 1/100 dilution staining Progesterone Receptor in human breast carcinoma by Immunohistochemistry, Paraffin-embedded tissue.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Western blot - Anti-Progesterone Receptor (phospho S190) antibody [EP1516Y] (ab75857)
    Western blot - Anti-Progesterone Receptor (phospho S190) antibody [EP1516Y] (ab75857)
    All lanes : Anti-Progesterone Receptor (phospho S190) antibody [EP1516Y] (ab75857) at 1/10000 dilution

    Lane 1 : T47D cell lysates, untreated
    Lane 2 : T47D cell lysates, treated with progesterone

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : goat anti-rabbit HRP at 1/2000 dilution

    Predicted band size: 99 kDa
    Observed band size: 118,99 kDa
    why is the actual band size different from the predicted?

  • Anti-Progesterone Receptor (phospho S190) antibody [EP1516Y] (ab75857)
    Anti-Progesterone Receptor (phospho S190) antibody [EP1516Y] (ab75857)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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