Anti-Progesterone Receptor (phospho S190) antibody [EP1516Y] (ab75857)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP1516Y] to Progesterone Receptor (phospho S190)
- Suitable for: WB, IHC-P
- Reacts with: Human
Overview
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Product name
Anti-Progesterone Receptor (phospho S190) antibody [EP1516Y]
See all Progesterone Receptor primary antibodies -
Description
Rabbit monoclonal [EP1516Y] to Progesterone Receptor (phospho S190) -
Host species
Rabbit -
Specificity
ab75857 detects Progesterone Receptor phophorylated at serine 190. -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P HumanWB Human -
Immunogen
A phospho specific peptide corresponding to residues surrounding serine 190 of human Progesterone Receptor
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Positive control
- T47D cell lysates, untreated or treated with progesterone; human breast carcinoma tissue.
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General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EP1516Y -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Progesterone Receptor (phospho S190) antibody [EP1516Y] (ab75857)
ab75857 at 1/100 dilution staining Progesterone Receptor in human breast carcinoma by Immunohistochemistry, Paraffin-embedded tissue.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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All lanes : Anti-Progesterone Receptor (phospho S190) antibody [EP1516Y] (ab75857) at 1/10000 dilution
Lane 1 : T47D cell lysates, untreated
Lane 2 : T47D cell lysates, treated with progesterone
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 99 kDa
Observed band size: 118,99 kDa why is the actual band size different from the predicted?
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