Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Progesterone Receptor antibody [YR85] - BSA and Azide free (ab206926)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [YR85] to Progesterone Receptor - BSA and Azide free
  • Suitable for: ICC, IHC-P, IP, WB, Flow Cyt
  • Reacts with: Human

Overview

  • Product name

    Anti-Progesterone Receptor antibody [YR85] - BSA and Azide free
    See all Progesterone Receptor primary antibodies

  • Description

    Rabbit monoclonal [YR85] to Progesterone Receptor - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC, IHC-P, IP, WB, Flow Cytmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Human breast carcinoma, T47D cell lysate. This antibody gave a positive result when used in the following formaldehyde fixed cell lines: DU145.

  • General notes

    ab206926 is the carrier-free version of ab32085 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab206926 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Progesterone Receptor antibody [YR85] - BSA and Azide free (ab206926)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Progesterone Receptor antibody [YR85] - BSA and Azide free (ab206926)

    Immunohistochemical analysis of progesterone receptor expression in paraffin embedded human breast carcinoma, using 1/100 ab32085.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab32085).

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Western blot - Anti-Progesterone Receptor antibody [YR85] - BSA and Azide free (ab206926)
    Western blot - Anti-Progesterone Receptor antibody [YR85] - BSA and Azide free (ab206926)
    Anti-Progesterone Receptor antibody [YR85] - BSA and Azide free (ab206926) + Human breast cancer lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051)

    Predicted band size: 98 kDa
    Observed band size: 135 kDa
    why is the actual band size different from the predicted?


    Exposure time: 3 minutes


    Blocking buffer and concentration: 5% NFDM/TBST

    Diluting buffer and concentration: 5% NFDM/TBST

  • Immunocytochemistry - Anti-Progesterone Receptor antibody [YR85] - BSA and Azide free (ab206926)
    Immunocytochemistry - Anti-Progesterone Receptor antibody [YR85] - BSA and Azide free (ab206926)

    Clone YR85 (ab206926) has been successfully conjugated by Abcam. This image was generated using Anti-Progesterone Receptor antibody [YR85] (Alexa Fluor® 488). Please refer to ab199224 for protocol details.

    ab199224 staining Progesterone Receptor in MCF7 cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab199224 at a 1/100 dilution (shown in green) and ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 594), at a 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    This product also gave a positive signal under the same testing conditions in MCF7 cells fixed with 100% methanol (5 min).

  • Immunocytochemistry - Anti-Progesterone Receptor antibody [YR85] - BSA and Azide free (ab206926)
    Immunocytochemistry - Anti-Progesterone Receptor antibody [YR85] - BSA and Azide free (ab206926)

    Clone YR85 (ab206926) has been successfully conjugated by Abcam. This image was generated using Anti-Progesterone Receptor antibody [YR85] (Alexa Fluor® 647). Please refer to ab199455 for protocol details.

    ab199455 staining Progesterone Receptor in MCF7 cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab199455 at a 1/100 dilution (shown in red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488), at a 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    This product also gave a positive signal under the same testing conditions in MCF7 cells fixed with 100% methanol (5 min).

  • Immunocytochemistry - Anti-Progesterone Receptor antibody [YR85] - BSA and Azide free (ab206926)
    Immunocytochemistry - Anti-Progesterone Receptor antibody [YR85] - BSA and Azide free (ab206926)

    ICC/IF image of ab32085 stained DU145 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab32085 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab32085).

  • Flow Cytometry - Anti-Progesterone Receptor antibody [YR85] - BSA and Azide free (ab206926)
    Flow Cytometry - Anti-Progesterone Receptor antibody [YR85] - BSA and Azide free (ab206926)

    Flow Cytometry analysis of T47D (human mammary gland ductal carcinoma) cells labeling Progesterone Receptor with purified ab32085 at 1:1100 dilution(1ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488)(ab150077)(1:2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black)(ab172730) was used as the isotype control, Cell without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab32085).

  • OI-RD Scanning - Anti-Progesterone Receptor antibody [YR85] - BSA and Azide free (ab206926)
    OI-RD Scanning - Anti-Progesterone Receptor antibody [YR85] - BSA and Azide free (ab206926)
    Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab32085).

  • Anti-Progesterone Receptor antibody [YR85] - BSA and Azide free (ab206926)
    Anti-Progesterone Receptor antibody [YR85] - BSA and Azide free (ab206926)

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