Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Gelsolin plasma antibody [EPR1941Y] - BSA and Azide free (ab247406)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR1941Y] to Gelsolin plasma - BSA and Azide free
  • Suitable for: WB, IHC-P, IP
  • Reacts with: Human

Overview

  • Product name

    Anti-Gelsolin plasma antibody [EPR1941Y] - BSA and Azide free
    See all Gelsolin plasma primary antibodies

  • Description

    Rabbit monoclonal [EPR1941Y] to Gelsolin plasma - BSA and Azide free

  • Host species

    Rabbit

  • Specificity

    The antibody only detects the isoform 1 of the protein (86kDa).

  • Tested applications

    Suitable for: WB, IHC-P, IPmore details
    Unsuitable for: Flow Cyt or ICC

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    ab247406 is the carrier-free version of ab75832 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab247406 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-Gelsolin plasma antibody [EPR1941Y] - BSA and Azide free (ab247406)
    Western blot - Anti-Gelsolin plasma antibody [EPR1941Y] - BSA and Azide free (ab247406)
    All lanes : Anti-Gelsolin antibody [EP1940Y] (ab134183) at 1/10000 dilution

    Lanes 1 & 5 & 9 : THP-1 (human acute monocytic leukemia) whole cell lysate
    Lanes 2 & 6 & 10 : THP-1 cultured medium
    Lanes 3 & 7 & 11 : MCF-7 (human breast adenocarcinoma) whole cell lysate
    Lanes 4 & 8 & 12 : Human Plasma

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 86 kDa
    Observed band size: 81/86 kDa
    why is the actual band size different from the predicted?


    Exposure time: 10 seconds


    This data was developed using ab75832, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    The secretory isoform of gelsolin is at 86kDa. The cytoplasmic isoforms of gelsolin are at 81kDa. Lanes 5-8: ab109014 detected both secretory and cytoplasmic isoforms. Lanes 9-12: ab134183 detected both secretory and cytoplasmic isoforms.

  • Western blot - Anti-Gelsolin plasma antibody [EPR1941Y] - BSA and Azide free (ab247406)
    Western blot - Anti-Gelsolin plasma antibody [EPR1941Y] - BSA and Azide free (ab247406)
    Anti-Gelsolin plasma antibody [EPR1941Y] (ab75832) at 1/50000 dilution + human plasma at 10 µg

    Secondary
    HRP labeled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 86 kDa
    Observed band size: 90 kDa why is the actual band size different from the predicted?



    This data was developed using ab75832, the same antibody clone in a different buffer formulation.

  • Immunoprecipitation - Anti-Gelsolin plasma antibody [EPR1941Y] - BSA and Azide free (ab247406)
    Immunoprecipitation - Anti-Gelsolin plasma antibody [EPR1941Y] - BSA and Azide free (ab247406)

    This data was developed using ab75832, the same antibody clone in a different buffer formulation.

    ab75832 Immunoprecipitating Gelsolin plasma in mouse embryo whole cell lysate. 2µg of capture antibody (ab75832) per 0.35mg of lysate was used. 10µg of plasma was incubated with primary antibody (Panel A: ab75832, Panel B: ab134183) at a dilution of 1/1000 and VeriBlot for IP Detection Reagent (HRP) ab131366 was used for detection at a dilution of 1/10000.

    Lane 1: Human plasma

    Lane 2: Human plasma

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab75832 in Human plasma.

    Exposure time: Panel A - 8 seconds, Panel B - 3 minutes.

    Blocking and dilution buffer: 5% NFDM/TBST.

    The additional band at 50 kDa is a rabbit IgG heavy chain.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Gelsolin plasma antibody [EPR1941Y] - BSA and Azide free (ab247406)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Gelsolin plasma antibody [EPR1941Y] - BSA and Azide free (ab247406)
    This data was developed using ab75832, the same antibody clone in a different buffer formulation.ab75832 at 1/100 dilution staining Gelsolin plasma in human colon tissue, using a HRP/AP polymerized secondary antibody. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
  • Anti-Gelsolin plasma antibody [EPR1941Y] - BSA and Azide free (ab247406)
    Anti-Gelsolin plasma antibody [EPR1941Y] - BSA and Azide free (ab247406)

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