RhoB Activation Assay Kit (ab211165)
Key features and details
- Sample type: Adherent cells, Suspension cells, Tissue
Overview
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Product name
RhoB Activation Assay Kit -
Sample type
Tissue, Adherent cells, Suspension cells -
Species reactivity
Reacts with: Mouse, Rat, Human -
Product overview
RhoB Activation Assay Kit (ab211165) uses Rhotekin RBD Agarose beads to selectively isolate and pull-down the active form of Rho from purified samples or endogenous lysates. Subsequently, the precipitated GTP-Rho is detected by western blot analysis using an anti-RhoB specific polyclonal antibody, which detects the human, mouse and rat protein.
Features: 1) non radioactive assay format; 2) fast results: 1 hour assay plus electrophoresis/blotting time; 3) includes Cdc42 positive control; 4) pink colored agarose beads for easy identification during washing and aspiration steps.
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Notes
Small GTP-binding proteins (or GTPases) are a family of proteins that serve as molecular regulators in signaling transduction pathways.
RhoB is a 21 kDa protein that regulates a variety of biological response pathways that include cell growth, cell transformation and tumor invasion. Like other small GTPases, RhoB cycles between an inactive GDP-bound form and an active GTP-bound form: in its active (GTP-bound) state, RhoB binds specifically to the Rho-binding domain (RBD) of Rhotekin to control downstream signaling cascades.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 20 tests ab211178 - Rhotekin RBD Agarose Beads 1 x 200µl 100X GTPyS 1 x 20µl 100X GDP 1 x 20µl 5X Assay/Lysis Buffer 4 x 2ml Anti-rhoB Rabbit polyclonal antibody 1 x 10µl RhoB immunoblot Positive Control 1 x 100µl -
Research areas
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Function
Mediates apoptosis in neoplastically transformed cells after DNA damage. Not essential for development but affects cell adhesion and growth factor signaling in transformed cells. Plays a negative role in tumorigenesis as deletion causes tumor formation. Involved in intracellular protein trafficking of a number of proteins. Targets PKN1 to endosomes and is involved in trafficking of the EGF receptor from late endosomes to lysosomes. Also required for stability and nuclear trafficking of AKT1/AKT which promotes endothelial cell survival during vascular development. -
Sequence similarities
Belongs to the small GTPase superfamily. Rho family. -
Post-translational
modificationsPrenylation specifies the subcellular location of RHOB. The farnesylated form is localized to the plasma membrane while the geranylgeranylated form is localized to the endosome. -
Cellular localization
Late endosome membrane. Cell membrane. Nucleus. Late endosomal membrane (geranylgeranylated form). Plasma membrane (farnesylated form). Also detected at the nuclear margin and in the nucleus. - Information by UniProt
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Alternative names
- Aplysia RAS-related homolog 6
- ARH6
- ARHB
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Database links
- Entrez Gene: 388 Human
- Entrez Gene: 11852 Mouse
- Entrez Gene: 64373 Rat
- Omim: 165370 Human
- SwissProt: P62745 Human
- SwissProt: P62746 Mouse
- SwissProt: P62747 Rat
- Unigene: 502876 Human
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Images
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RhoB Activation Assay Kit (ab211165)
RhoB positive control (recombinant human RhoB from E. coli, partially purified) detected by immunoblot.
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RhoB Activation Assay Kit (ab211165)Dot blot that demonstrates specificity of anti-RhoB rabbit antibody.