Anti-DIAPH3 (phospho T1061) antibody (ab202281)
Key features and details
- Rabbit polyclonal to DIAPH3 (phospho T1061)
- Suitable for: WB
- Reacts with: Recombinant fragment
- Isotype: IgG
Overview
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Product name
Anti-DIAPH3 (phospho T1061) antibody
See all DIAPH3 primary antibodies -
Description
Rabbit polyclonal to DIAPH3 (phospho T1061) -
Host species
Rabbit -
Specificity
The immunogen sequence for ab202281 has low homology to other formins. -
Tested Applications & Species
See all applications and species dataApplication Species WB Recombinant fragment
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Immunogen
Synthetic peptide corresponding to Mouse DIAPH3 (phospho T1061) conjugated to Keyhole Limpet Haemocyanin (KLH). A phospho specific synthetic peptide corresponding to residues surrounding threonine 1061. This peptide sequence is identical in rat and has 2 amino acid differences to Human mDia2.
Database link: Q9Z207 -
Positive control
- Monkey COS7 cells transfected with recombinant DIAPH3, untreated or treated with calyculin A.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituents: 50% Glycerol, 49% PBS, 0.1% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab202281 was affinity purified using DIAPH3 (Thr-1061) peptide (without carrier). -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-DIAPH3 (phospho T1061) antibody (ab202281)All lanes : Anti-DIAPH3 (phospho T1061) antibody (ab202281) at 1/1000 dilution
Lane 1 : monkey COS7 cells transfected with recombinant DIAPH3
Lane 2 : monkey COS7 cells transfected with recombinant DIAPH3 treated with Calyculin A and alkaline phosphatase
Lane 3 : monkey COS7 cells transfected with recombinant DIAPH3 treated with calyculin A
Lane 4 : monkey COS7 cells transfected with recombinant DIAPH3 (T1061A)
Predicted band size: 134 kDaThe lower blot was probed with anti-DDDDK antibody.
