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Anti-DIAPH3 (phospho T1061) antibody (ab202281)

Key features and details

  • Rabbit polyclonal to DIAPH3 (phospho T1061)
  • Suitable for: WB
  • Reacts with: Recombinant fragment
  • Isotype: IgG

Overview

  • Product name

    Anti-DIAPH3 (phospho T1061) antibody
    See all DIAPH3 primary antibodies

  • Description

    Rabbit polyclonal to DIAPH3 (phospho T1061)

  • Host species

    Rabbit

  • Specificity

    The immunogen sequence for ab202281 has low homology to other formins.

  • Tested Applications & Species

    Application Species
    WB
    Recombinant fragment
    See all applications and species data

  • Immunogen

    Synthetic peptide corresponding to Mouse DIAPH3 (phospho T1061) conjugated to Keyhole Limpet Haemocyanin (KLH). A phospho specific synthetic peptide corresponding to residues surrounding threonine 1061. This peptide sequence is identical in rat and has 2 amino acid differences to Human mDia2.
    Database link: Q9Z207

  • Positive control

    • Monkey COS7 cells transfected with recombinant DIAPH3, untreated or treated with calyculin A.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    Preservative: 0.05% Sodium azide
    Constituents: 50% Glycerol, 49% PBS, 0.1% BSA
  • Concentration information loading...

  • Purity

    Immunogen affinity purified

  • Purification notes

    ab202281 was affinity purified using DIAPH3 (Thr-1061) peptide (without carrier).

  • Clonality

    Polyclonal

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-DIAPH3 (phospho T1061) antibody (ab202281)
    Western blot - Anti-DIAPH3 (phospho T1061) antibody (ab202281)
    All lanes : Anti-DIAPH3 (phospho T1061) antibody (ab202281) at 1/1000 dilution

    Lane 1 : monkey COS7 cells transfected with recombinant DIAPH3
    Lane 2 : monkey COS7 cells transfected with recombinant DIAPH3 treated with Calyculin A and alkaline phosphatase
    Lane 3 : monkey COS7 cells transfected with recombinant DIAPH3 treated with calyculin A
    Lane 4 : monkey COS7 cells transfected with recombinant DIAPH3 (T1061A)

    Predicted band size: 134 kDa



    The lower blot was probed with anti-DDDDK antibody. 

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