Human Procalcitonin Matched Antibody Pair Kit (ab222276)
Key features and details
- Unlabeled capture antibody, biotin-labeled detection antibody and calibrated protein standard
- For economical ELISA and ELISA-based assay development
- Reacts with: Human
- Range: 0.98 pg/ml - 125 pg/ml
Overview
-
Product name
Human Procalcitonin Matched Antibody Pair Kit
See all Procalcitonin kits -
Detection method
Colorimetric -
Assay type
ELISA set -
Sensitivity
0.2 pg/ml -
Range
0.98 pg/ml - 125 pg/ml -
Species reactivity
Reacts with: Human -
Product overview
Human Procalcitonin Matched Antibody Pair Kits include a capture and a biotinylated detector antibody pair, along with a calibrated protein standard, suitable for sandwich ELISA. The Matched Antibody Pair Kit can be used to quantify native and recombinant human Procalcitonin.
Optimization of the kit reagents to sample type, immunoassay format or instrumentation may be required. Guidelines for use of this kit in a standard 96-well microplate sandwich ELISA using HRP/TMB system of colorimetric detection is described in this assay procedure for the purposes of quantification.
Protocol information and tips on the use of the Matched Antibody Pair kits for sandwich ELISA can be found on our website. An accessory pack can be purchased which includes buffer reagents required to perform 10 x 96-well plate sandwich ELISAs (ab210905).
For additional information on the performance of the antibody pair used in this kit, please see our equivalent SimpleStep ELISA kit ab221828. Please note that while the antibody pair is the same provided in the corresponding SimpleStep ELISA Kit, due to differences in their formulation, this antibody pair cannot be used with the consumables provided with our SimpleStep ELISA Kits.
-
Tested applications
Suitable for: ELISAmore details -
Platform
Reagents
Properties
-
Storage instructions
Store at -20°C. Please refer to protocols. -
Components 10 x 96 tests 5 x 96 tests Human Procalcitonin Capture Antibody 2 x 50µg 1 x 50µg Human Procalcitonin Detector Antibody 2 x 12.5µg 1 x 12.5µg Human Procalcitonin Lyophilized Protein 2 vials 1 vial -
Research areas
-
Relevance
Procalcitonin (PCT) is a 116 amino acid residue peptide with molecular weight of about 13 kDa. PCT itself has no known hormonal activity. PCT belongs to a group of related proteins including calcitonin gene-related peptides I and II, amylin, adrenomodulin and calcitonin (CAPA peptide family). PCT, like other peptides of CAPA family, appears from the common precursor pre-procalcitonin consisting of 141 amino acids by removal of 25 amino acids from the N-terminus. PCT undergoes successive cleavages to form three molecules: N-terminal fragment (55 a.a.), calcitonin (32 a.a.) and katacalcin (21 a.a.). Under normal metabolic conditions, PCT is only present in the C cells of the thyroid gland. In bacterial infection and sepsis, however, intact PCT is found in the blood and, more importantly, its level is related to the severity of bacterial sepsis. Today, PCT is considered to be one of the earliest and most specific markers of sepsis. -
Cellular localization
Secreted -
Alternative names
- CALC1
- CALCA
- calcitonin related polypeptide alpha
see all -
Database links
- Entrez Gene: 796 Human
- Omim: 114130 Human
- SwissProt: P01258 Human
Images
-
Human Procalcitonin standard curve
Standard calibration curve. Background subtracted values are graphed.
-
Sandwich ELISA - Human Procalcitonin Matched Antibody Pair Kit (ab222276)To learn more about the advantages of recombinant antibodies see here.
