Astana Biomed Group, an authorized Abcam distributor in Central Asia

Human BTK Antibody Pair - BSA and Azide free (ab253476)

Key features and details

  • Unconjugated capture and detector antibodies
  • Adaptable to any antibody pair-based assay format
  • Antibody concentration ~ 1 mg/ml
  • BSA and azide free buffer - ready for conjugation
  • Reacts with: Human

Overview

  • Product name

    Human BTK Antibody Pair - BSA and Azide free
    See all BTK kits

  • Assay type

    ELISA set

  • Range

    156.25 pg/ml - 10000 pg/ml

  • Species reactivity

    Reacts with: Human

  • Product overview

    Both capture and detector antibodies are recombinant rabbit monoclonal antibodies delivering consistent, specific, and sensitive results.


    The Antibody Pair can be used to quantify Human BTK. BSA and Azide free antibody pairs include unconjugated capture and detector antibodies suitable for sandwich ELISAs. The antibodies are provided at an approximate concentration of 1 mg/ml as measured by the protein A280 method. The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type.


    For additional information on the performance of the antibody pair, see the equivalent SimpleStep ELISA® Kit (ab277089), which uses the same antibodies. However, due to differences in their formulation, this antibody pair cannot be used with the consumables provided with our SimpleStep ELISA Kits. Please note that the range provided for the pairs is only an estimation based on the performance of the related product using the same antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody pair in your assay.


    Download SDS here.

  • Tested applications

    Suitable for: Sandwich ELISAmore details

  • Platform

    Reagents

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.

  • Carrier free

    Yes
  • Components 10 x 96 tests
    Human BTK Capture Antibody (unconjugated) 1 x 100µg
    Human BTK Detector Antibody (unconjugated) 1 x 100µg

  • Research areas

  • Function

    Plays a crucial role in B-cell ontogeny. Transiently phosphorylates GTF2I on tyrosine residues in response to B-cell receptor cross-linking. Required for the formation of functional ARID3A DNA-binding complexes.

  • Involvement in disease

    Defects in BTK are the cause of X-linked agammaglobulinemia (XLA) [MIM:300755]; also known as X-linked agammaglobulinemia type 1 (AGMX1) or immunodeficiency type 1 (IMD1). XLA is a humoral immunodeficiency disease which results in developmental defects in the maturation pathway of B-cells. Affected boys have normal levels of pre-B-cells in their bone marrow but virtually no circulating mature B-lymphocytes. This results in a lack of immunoglobulins of all classes and leads to recurrent bacterial infections like otitis, conjunctivitis, dermatitis, sinusitis in the first few years of life, or even some patients present overwhelming sepsis or meningitis, resulting in death in a few hours. Treatment in most cases is by infusion of intravenous immunoglobulin.
    Defects in BTK may be the cause of X-linked hypogammaglobulinemia and isolated growth hormone deficiency (XLA-IGHD) [MIM:307200]; also known as agammaglobulinemia and isolated growth hormone deficiency or Fleisher syndrome or isolated growth hormone deficiency type 3 (IGHD3). In rare cases XLA is inherited together with isolated growth hormone deficiency (IGHD).

  • Sequence similarities

    Belongs to the protein kinase superfamily. Tyr protein kinase family. TEC subfamily.
    Contains 1 Btk-type zinc finger.
    Contains 1 PH domain.
    Contains 1 protein kinase domain.
    Contains 1 SH2 domain.
    Contains 1 SH3 domain.

  • Post-translational
    modifications

    Autophosphorylated on Tyr-223 and Tyr-551. Phosphorylation of Tyr-223 may create a docking site for a SH2 containing protein.

  • Cellular localization

    Cytoplasm. Membrane. Nucleus.
  • Information by UniProt

  • Alternative names

    • Agammaglobulinaemia tyrosine kinase
    • AGMX 1
    • AGMX1
    • AT
    • ATK
    • B cell progenitor kinase
    • B-cell progenitor kinase
    • BPK
    • Bruton agammaglobulinemia tyrosine kinase
    • Bruton tyrosine kinase
    • Bruton’s Tyrosine Kinase
    • Btk
    • BTK_HUMAN
    • dominant-negative kinase-deficient Brutons tyrosine kinase
    • IMD 1
    • IMD1
    • MGC126261
    • MGC126262
    • OTTHUMP00000063593
    • PSCTK 1
    • PSCTK1
    • truncated Bruton agammaglobulinemia tyrosine kinase
    • Tyrosine protein kinase BTK
    • Tyrosine-protein kinase BTK
    • tyrosine-protein kinase BTK isoform (lacking exon 14
    • XLA
    see all

  • Database links

    Images

    • Sandwich ELISA - Human BTK Antibody Pair - BSA and Azide free (ab253476)
      Sandwich ELISA - Human BTK Antibody Pair - BSA and Azide free (ab253476)
      To learn more about the advantages of recombinant antibodies see here.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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