Human Procalcitonin ELISA Kit (ab221828)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 1.51 pg/ml
- Range: 6.25 pg/ml - 400 pg/ml
- Sample type: Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Milk, Saliva, Serum, Urine
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human Procalcitonin ELISA Kit
See all Procalcitonin kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Urine 8 2.9% Inter-assay Sample n Mean SD CV% Urine 3 4.4% -
Sample type
Cell culture supernatant, Saliva, Milk, Urine, Serum, Hep Plasma, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
1.51 pg/ml -
Range
6.25 pg/ml - 400 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Saliva 94 84% - 104% Milk 79 75% - 88% Urine 90 88% - 92% Serum 79 74% - 82% Cell culture media 100 99% - 101% Hep Plasma 93 90% - 100% EDTA Plasma 78 75% - 82% Cit plasma 83 79% - 88% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human
Does not react with: Cow -
Product overview
Human Procalcitonin ELISA Kit (ab221828) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Procalcitonin protein in cell culture supernatant, cit plasma, edta plasma, hep plasma, milk, saliva, serum, and urine. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Procalcitonin with 1.51 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
Human Procalcitonin is a 116-amino acid precursor protein of the peptide hormone called Calcitonin coded by the gene CALCA. Procalcitonin is mainly produced by the C-cells of the thyroid and certain endocrine cells of the lung. Under normal expression conditions, Procalcitonin is cleaved into three specific fragments: an N-terminal residue, Calcitonin, and Katacalcin. Levels of unprocessed Procalcitonin have been shown to rise significantly after bacterial infection, trauma, and shock. Calcitonin regulates the blood phosphate and calcium levels by promoting incorporation into bones. Populations with chronic kidney disease have been shown to have higher procalcitonin levels. The antibodies used in this product were raised to the propeptide sequence residues 26-82. The standard protein in this product has been mass calibrated to a full-length procalcitonin (residues 26-141).
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Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Human Procalcitonin Capture Antibody 1 x 600µl 10X Human Procalcitonin Detector Antibody 1 x 600µl Human Procalcitonin Lyophilized Recombinant Protein 2 vials Antibody Diluent 4BI 1 x 6ml 10X Wash Buffer PT (ab206977) 1 x 20ml TMB Development Solution 1 x 12ml Stop Solution 1 x 12ml Sample Diluent NS (ab193972) 1 x 50ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Plate Seals 1 unit -
Research areas
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Relevance
Procalcitonin (PCT) is a 116 amino acid residue peptide with molecular weight of about 13 kDa. PCT itself has no known hormonal activity. PCT belongs to a group of related proteins including calcitonin gene-related peptides I and II, amylin, adrenomodulin and calcitonin (CAPA peptide family). PCT, like other peptides of CAPA family, appears from the common precursor pre-procalcitonin consisting of 141 amino acids by removal of 25 amino acids from the N-terminus. PCT undergoes successive cleavages to form three molecules: N-terminal fragment (55 a.a.), calcitonin (32 a.a.) and katacalcin (21 a.a.). Under normal metabolic conditions, PCT is only present in the C cells of the thyroid gland. In bacterial infection and sepsis, however, intact PCT is found in the blood and, more importantly, its level is related to the severity of bacterial sepsis. Today, PCT is considered to be one of the earliest and most specific markers of sepsis. -
Cellular localization
Secreted -
Alternative names
- CALC1
- CALCA
- calcitonin related polypeptide alpha
see all -
Database links
- Entrez Gene: 796 Human
- Omim: 114130 Human
- SwissProt: P01258 Human
Images
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Other - Human Procalcitonin ELISA Kit (ab221828)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of human Procalcitonin standard curve.Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of spiked Procalcitonin in human serum and plasma samples.The concentrations of Procalcitonin were measured in duplicates, interpolated from the Procalcitonin standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 50%, plasma (citrate) 50%, plasma (EDTA) 50%, and plasma (heparin) 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Procalcitonin concentration was determined to be 335 pg/mL in neat serum, 351 pg/mL in neat plasma (citrate), 424 pg/mL in neat plasma (EDTA) and 405 pg/mL in neat plasma (heparin).
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Interpolated concentrations of native Procalcitonin in human serum and plasma samples.The concentrations of Procalcitonin were measured in duplicates, interpolated from the Procalcitonin standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 50%, plasma (citrate) 50%, plasma (EDTA) 50%, and plasma (heparin) 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Procalcitonin concentration was determined to be 54 pg/mL in neat serum, 38 pg/mL in neat plasma (citrate), 114 pg/mL in neat plasma (EDTA) and 43 pg/mL in neat plasma (heparin).
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Interpolated concentrations of native Procalcitonin in human urine, milk, and spiked Procalcitonin in human saliva samples.The concentrations of Procalcitonin were measured in duplicates, interpolated from the Procalcitonin standard curves and corrected for sample dilution. Undiluted samples are as follows: urine 25%, milk 50%, and saliva 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Procalcitonin concentration was determined to be 1,555 pg/mL in neat urine, 154 pg/mL in neat milk, and 338 pg/mL in neat saliva.
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Serum from ten individual healthy human male donors was measured in duplicate.Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Procalcitonin concentration in neat serum was determined to be 139 pg/mL with a range of 33 – 438 pg/mL.
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Sandwich ELISA - Human Procalcitonin ELISA Kit (ab221828)To learn more about the advantages of recombinant antibodies see here.
