Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-SIK1 (phospho T182) + SIK2 (phospho T175) + SIK3 antibody [EPR19196] - BSA and Azide free (ab271963)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR19196] to SIK1 (phospho T182) + SIK2 (phospho T175) + SIK3 (phospho T163) - BSA and Azide free
  • Suitable for: Dot blot, WB
  • Reacts with: Human

Overview

  • Product name

    Anti-SIK1 (phospho T182) + SIK2 (phospho T175) + SIK3 antibody [EPR19196] - BSA and Azide free

  • Description

    Rabbit monoclonal [EPR19196] to SIK1 (phospho T182) + SIK2 (phospho T175) + SIK3 (phospho T163) - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Dot blot, WBmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HEK-293T transfected with human Snf1lk or Snf1lk2 or SIK3 (WT) whole cell lysate.

  • General notes

    This product was previously labelled as Snf1lk2, Snf1lk+Snf1lk2+SIK3.

    ab271963 is the carrier-free version of ab199474. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Dot Blot - Anti-SIK1+SIK2+SIK3 antibody [EPR19196] - BSA and Azide free (ab271963)
    Dot Blot - Anti-SIK1+SIK2+SIK3 antibody [EPR19196] - BSA and Azide free (ab271963)

    Dot blot analysis of Snf1lk2 (phospho T175) labeled with ab199474 at 1/1,000 dilution.

    Lane 1: Snf1lk2 (phospho T175) peptide

    Lane 2: Snf1lk2 non-phospho peptide

    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100,000 dilution was used as secondary antibody.

    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time: 3 minutes.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab199474).
  • Anti-SIK1 (phospho T182) + SIK2 (phospho T175) + SIK3 antibody [EPR19196] - BSA and Azide free (ab271963)
    Anti-SIK1 (phospho T182) + SIK2 (phospho T175) + SIK3 antibody [EPR19196] - BSA and Azide free (ab271963)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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