Anti-mTOR antibody (ab265599)
Key features and details
- Rabbit polyclonal to mTOR
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-mTOR antibody
See all mTOR primary antibodies -
Description
Rabbit polyclonal to mTOR -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat
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Immunogen
Synthetic peptide within Human mTOR aa 1675-1725. The exact sequence is proprietary.
Database link: P42345 -
Positive control
- WB: HeLa and HEK-293T whole cell lysate. IP: HeLa whole cell lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-mTOR antibody (ab265599)All lanes : Anti-mTOR antibody (ab265599) at 0.1 µg/ml
Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lysates/proteins at 50 µg per lane.
Predicted band size: 289 kDa
Exposure time: 3 minutesCells prepared using NETN lysis buffer.
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Immunoprecipitation - Anti-mTOR antibody (ab265599)mTOR was immunoprecipitated from 1mg of HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate with ab265599 at 6 µg per reaction. Western blot was performed from the immunoprecipitate using ab265599 at 1 µg/ml.
Lane 1: ab265599 (Lot 1) IP in HeLa whole cell lysate.
Lane 2: ab265599 (Lot 2) IP in HeLa whole cell lysate.
Lane 3: Control IgG.Exposure time: 3 mins.
Different lots were used in lane 1 and lane 2.
Cells prepared using NETN lysis buffer.
