Antibodies, Proteins, Kits and Reagents for Life Science

318 288 ₸ Product size

100 µl 318 288 ₸

Order now and get it on Tuesday March 02, 2021

Anti-SH3PX1/SNX9 antibody [EPR14399] (ab181856)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR14399] to SH3PX1/SNX9
Suitable for: WB, IHC-P, ICC/IF, Flow Cyt, IP
Knockout validated
Reacts with: Mouse, Human

Product name

Anti-SH3PX1/SNX9 antibody [EPR14399]
See all SH3PX1/SNX9 primary antibodies
Description

Rabbit monoclonal [EPR14399] to SH3PX1/SNX9
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC/IF	Human
IHC-P	Human
IP	Human
WB	Human

See all applications and species data

Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- HeLa and HCT-116 cells and cell lysates; Human colon tissue.
General notes
This product was previously labelled as SH3PX1

This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 0.05% BSA, 40% Glycerol (glycerin, glycerine)
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR14399
Isotype

IgG
Research areas

Western blot - Anti-SH3PX1/SNX9 antibody [EPR14399] (ab181856)

Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: SH3PX1/SNX9 knockout HAP1 cell lysate (20 µg)
Lane 3: HeLa cell lysate (20 µg)
Lane 4: HCT116 cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab181856 observed at 75 kDa. Red - loading control, ab8245, observed at 37 kDa.

ab181856 was shown to specifically react with SH3PX1/SNX9 when SH3PX1/SNX9 knockout samples were used. Wild-type and SH3PX1/SNX9 knockout samples were subjected to SDS-PAGE. ab181856 and ab8245 (loading control to GAPDH) were diluted 1/1000 and 1/10000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773)
and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.
Immunoprecipitation - Anti-SH3PX1/SNX9 antibody [EPR14399] (ab181856)

Western blot analysis of HepG2 cell lysate immunoprecipitated using ab181856 at 1/50 dilution. Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate secondary antibody was used at 1/1000 dilution.
Flow Cytometry - Anti-SH3PX1/SNX9 antibody [EPR14399] (ab181856)

Flow cytometric analysis of 2% paraformaldehyde-fixed HeLa cells labeling SH3PX1/SNX9 with ab181856 at 1/10 dilution (red) compared to a Rabbit IgG monoclonal isotype control (green), followed by Goat anti rabbit IgG (FITC) secondary antibody at 1/150 dilution.
Immunocytochemistry/ Immunofluorescence - Anti-SH3PX1/SNX9 antibody [EPR14399] (ab181856)

Immunofluorescent analysis of 4% paraformaldehyde-fixed HCT-116 cells labeling SH3PX1/SNX9 with ab181856 at 1/100 dilution, followed by Goat anti rabbit IgG (Alexa Fluor®488) secondary antibody at 1/200 dilution. Counter stained with Dapi.
Immunocytochemistry/ Immunofluorescence - Anti-SH3PX1/SNX9 antibody [EPR14399] (ab181856)

Immunofluorescent analysis of acetone-fixed HeLa cells labeling SH3PX1/SNX9 with ab181856 at 1/100 dilution, followed by Goat anti rabbit IgG (Alexa Fluor®488) secondary antibody at 1/200 dilution. Counter stained with Dapi.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SH3PX1/SNX9 antibody [EPR14399] (ab181856)

Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling SH3PX1/SNX9 with ab181856 at 1/250 dilution, followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
Western blot - Anti-SH3PX1/SNX9 antibody [EPR14399] (ab181856)

Anti-SH3PX1/SNX9 antibody [EPR14399] (ab181856) at 1/10000 dilution + HCT-116 cell lysate at 20 µg

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Predicted band size: 67 kDa
Observed band size: 78 kDa
why is the actual band size different from the predicted?
Western blot - Anti-SH3PX1/SNX9 antibody [EPR14399] (ab181856)

Anti-SH3PX1/SNX9 antibody [EPR14399] (ab181856) at 1/50000 dilution + HeLa cell lysate at 20 µg

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Predicted band size: 67 kDa
Observed band size: 78 kDa why is the actual band size different from the predicted?
Anti-SH3PX1/SNX9 antibody [EPR14399] (ab181856)

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