Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-SH3PX1/SNX9 antibody [EPR14399] (ab181856)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR14399] to SH3PX1/SNX9
  • Suitable for: WB, IHC-P, ICC/IF, Flow Cyt, IP
  • Knockout validated
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-SH3PX1/SNX9 antibody [EPR14399]
    See all SH3PX1/SNX9 primary antibodies

  • Description

    Rabbit monoclonal [EPR14399] to SH3PX1/SNX9

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Human
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • HeLa and HCT-116 cells and cell lysates; Human colon tissue.

  • General notes

     

     

     This product was previously labelled as SH3PX1

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-SH3PX1/SNX9 antibody [EPR14399] (ab181856)
    Western blot - Anti-SH3PX1/SNX9 antibody [EPR14399] (ab181856)

    Lane 1: Wild-type HAP1 cell lysate (20 µg)
    Lane 2: SH3PX1/SNX9 knockout HAP1 cell lysate (20 µg)
    Lane 3: HeLa cell lysate (20 µg)
    Lane 4: HCT116 cell lysate (20 µg)
    Lanes 1 - 4: Merged signal (red and green). Green - ab181856 observed at 75 kDa. Red - loading control, ab8245, observed at 37 kDa.

    ab181856 was shown to specifically react with SH3PX1/SNX9 when SH3PX1/SNX9 knockout samples were used. Wild-type and SH3PX1/SNX9 knockout samples were subjected to SDS-PAGE. ab181856 and ab8245 (loading control to GAPDH) were diluted 1/1000 and 1/10000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773)
    and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

  • Immunoprecipitation - Anti-SH3PX1/SNX9 antibody [EPR14399] (ab181856)
    Immunoprecipitation - Anti-SH3PX1/SNX9 antibody [EPR14399] (ab181856)

    Western blot analysis of HepG2 cell lysate immunoprecipitated using ab181856 at 1/50 dilution. Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate secondary antibody was used at 1/1000 dilution.

  • Flow Cytometry - Anti-SH3PX1/SNX9 antibody [EPR14399] (ab181856)
    Flow Cytometry - Anti-SH3PX1/SNX9 antibody [EPR14399] (ab181856)

    Flow cytometric analysis of 2% paraformaldehyde-fixed HeLa cells labeling SH3PX1/SNX9 with ab181856 at 1/10 dilution (red) compared to a Rabbit IgG monoclonal isotype control (green), followed by Goat anti rabbit IgG (FITC) secondary antibody at 1/150 dilution.

  • Immunocytochemistry/ Immunofluorescence - Anti-SH3PX1/SNX9 antibody [EPR14399] (ab181856)
    Immunocytochemistry/ Immunofluorescence - Anti-SH3PX1/SNX9 antibody [EPR14399] (ab181856)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed HCT-116 cells labeling SH3PX1/SNX9 with ab181856 at 1/100 dilution, followed by Goat anti rabbit IgG (Alexa Fluor®488) secondary antibody at 1/200 dilution. Counter stained with Dapi.

  • Immunocytochemistry/ Immunofluorescence - Anti-SH3PX1/SNX9 antibody [EPR14399] (ab181856)
    Immunocytochemistry/ Immunofluorescence - Anti-SH3PX1/SNX9 antibody [EPR14399] (ab181856)

    Immunofluorescent analysis of acetone-fixed HeLa cells labeling SH3PX1/SNX9 with ab181856 at 1/100 dilution, followed by Goat anti rabbit IgG (Alexa Fluor®488) secondary antibody at 1/200 dilution. Counter stained with Dapi.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SH3PX1/SNX9 antibody [EPR14399] (ab181856)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SH3PX1/SNX9 antibody [EPR14399] (ab181856)

    Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling SH3PX1/SNX9 with ab181856 at 1/250 dilution, followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Western blot - Anti-SH3PX1/SNX9 antibody [EPR14399] (ab181856)
    Western blot - Anti-SH3PX1/SNX9 antibody [EPR14399] (ab181856)
    Anti-SH3PX1/SNX9 antibody [EPR14399] (ab181856) at 1/10000 dilution + HCT-116 cell lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 67 kDa
    Observed band size: 78 kDa
    why is the actual band size different from the predicted?

  • Western blot - Anti-SH3PX1/SNX9 antibody [EPR14399] (ab181856)
    Western blot - Anti-SH3PX1/SNX9 antibody [EPR14399] (ab181856)
    Anti-SH3PX1/SNX9 antibody [EPR14399] (ab181856) at 1/50000 dilution + HeLa cell lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

    Predicted band size: 67 kDa
    Observed band size: 78 kDa why is the actual band size different from the predicted?

  • Anti-SH3PX1/SNX9 antibody [EPR14399] (ab181856)
    Anti-SH3PX1/SNX9 antibody [EPR14399] (ab181856)

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