Antibodies, Proteins, Kits and Reagents for Life Science

284 784 ₸ Product size

100 µl 284 784 ₸

Order now and get it on Tuesday March 02, 2021

Anti-FOXC1 antibody [EPR20678] (ab223850)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR20678] to FOXC1
Suitable for: IP, WB, IHC-P, Flow Cyt
Reacts with: Mouse, Rat, Human

Product name

Anti-FOXC1 antibody [EPR20678]
See all FOXC1 primary antibodies
Description

Rabbit monoclonal [EPR20678] to FOXC1
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
IHC-P	Human
IP	Human
WB	Mouse Rat Human

See all applications and species data

Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: HEK-293T, HeLa, 4T1, MDA-MB-231, MDA-MB-435S, PC-12 and NIH/3T3 whole cell lysates; Human fetal spleen lysate. IHC-P: Human gastric cancer, pancreatic cancer and basal-like breast cancer tissues. Flow Cyt: HEK-293T cells. IP: HeLa whole cell lysate.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR20678
Isotype

IgG
Research areas

Western blot - Anti-FOXC1 antibody [EPR20678] (ab223850)

All lanes : Anti-FOXC1 antibody [EPR20678] (ab223850) at 1/1000 dilution

Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 20 µg
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 20 µg
Lane 3 : 4T1 (mouse mammary gland carcinoma epithelial cell line) whole cell lysate at 10 µg
Lane 4 : MDA-MB-231 (human breast adenocarcinoma cell line) whole cell lysate at 20 µg
Lane 5 : MDA-MB-435S (human ductal carcinoma cell line) whole cell lysate at 20 µg
Lane 6 : PC-12 (rat adrenal gland pheochromocytoma cell line) whole cell lysate at 10 µg
Lane 7 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 10 µg
Lane 8 : Human fetal spleen lysate at 10 µg

Secondary
Lanes 1-7 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution (Goat Anti-Rabbit IgG, (H+L) Peroxidase conjugated)
Lane 8 : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/4000 dilution (Goat Anti-Rabbit IgG, (H+L) Peroxidase conjugated)

Predicted band size: 56 kDa
Observed band size: 70 kDa
why is the actual band size different from the predicted?

Exposure time : Lane 1: 15 seconds; Lanes 2-3 & 6-8: 3 minutes; Lanes 4-5: 1 minute.

Blocking/Dilution buffer: 5% NFDM/TBST.

The molecular mass is consistent with what has been described in the literature (PMID: 27708239).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXC1 antibody [EPR20678] (ab223850)

Immunohistochemical analysis of paraffin-embedded human gastric cancer tissue labeling FOXC1 with ab223850 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Nuclear staining in human gastric cancer is observed(PMID:24329718). Counter stained with hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXC1 antibody [EPR20678] (ab223850)

Immunohistochemical analysis of paraffin-embedded human pancreatic cancer tissue labeling FOXC1 with ab223850 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Nuclear staining in human pancreatic cancer is observed(PMID:23242609). Counter stained with hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXC1 antibody [EPR20678] (ab223850)

Immunohistochemical analysis of paraffin-embedded human basal-like breast cancer tissue labeling FOXC1 with ab223850 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Nuclear staining in human basal-like breast cancer (PMID:27708239; PMID: 20406990). Counter stained with hematoxylin.

Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Flow Cytometry - Anti-FOXC1 antibody [EPR20678] (ab223850)

Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell line labeling FOXC1 with ab223850 at 1/500 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) at 1/2000 dilution was used as the secondary antibody.
Immunoprecipitation - Anti-FOXC1 antibody [EPR20678] (ab223850)

FOXC1 was immunoprecipitated from 0.35 mg of HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate with ab223850 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab223850 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

Lane 1: HeLa whole cell lysate 10 µg (Input).

Lane 2: ab223850 IP in HeLa whole cell lysate.

Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab223850 in HeLa whole cell lysate.

Blocking and dilution buffer and concentration: 5% NFDM/TBST.

Exposure time: 3 minutes.

The molecular mass is consistent with what has been described in the literature (PMID: 22493429).
Anti-FOXC1 antibody [EPR20678] (ab223850)

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