Anti-FOXC1 antibody (ab226219)
Key features and details
- Rabbit polyclonal to FOXC1
- Suitable for: WB, IP, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-FOXC1 antibody
See all FOXC1 primary antibodies -
Description
Rabbit polyclonal to FOXC1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IP, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human FOXC1 aa 375-425. The exact sequence is proprietary. (NP_001444.2).
Database link: Q12948 -
Positive control
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab226219 was affinity purified using an epitope specific to FOXC1 immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
-
Western blot - Anti-FOXC1 antibody (ab226219)All lanes : Anti-FOXC1 antibody (ab226219) at 0.04 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg
Lane 4 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 57 kDa
Exposure time: 10 seconds
-
Immunoprecipitation - Anti-FOXC1 antibody (ab226219)FOXC1 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab226219 at 6 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab226219 at 1 µg/ml.
Lane 1: ab226219 IP in HeLa whole cell lysate.
Lane 2: Control IgG IP in HeLa whole cell lysate.
Detection: Chemiluminescence with exposure time of 10 seconds.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FOXC1 antibody (ab226219)Formalin-fixed, paraffin-embedded human breast carcinoma tissue stained for FOXc1 using ab226219 at 1/200 dilution in immunohistochemical analysis.
Detection: DAB staining. Counterstain: Hematoxylin (blue).
