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Epigenetics and Nuclear Signaling Transcription Domain Families Forkhead Box

Anti-FoxJ1 antibody (ab108452)

Price and availability

247 929 ₸

Availability

Order now and get it on Thursday February 25, 2021

Anti-FoxJ1 antibody (ab108452)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Rabbit polyclonal to FoxJ1
  • Suitable for: WB
  • Reacts with: Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-FoxJ1 antibody
    See all FoxJ1 primary antibodies
  • Description

    Rabbit polyclonal to FoxJ1
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • This antibody gave a positive signal in A549 whole cell lysate.
  • General notes

     This product was previously labelled as HFH4

     

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS

    Batches of this product that have a concentration
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Transcription
    • Domain Families
    • Forkhead Box
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Domain Families
    • Forkhead Box
    • Other FOXes

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab108452 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
WB
Human
All applications
Mouse
Rat
Horse
Cow
Dog
Chimpanzee
Macaque monkey
Gorilla
Orangutan
Application Abreviews Notes
WB
1/250. Detects a band of approximately 49 kDa (predicted molecular weight: 45 kDa).
Notes
WB
1/250. Detects a band of approximately 49 kDa (predicted molecular weight: 45 kDa).

Target

  • Function

    May play an important role in cell fate determination during lung development and in spermatogenesis.
  • Tissue specificity

    Testis, oviduct, lung and brain cortex.
  • Involvement in disease

    Genetic variations in FOXJ1 may be associated with susceptibility to allergic rhinitis (ALRH) [MIM:607154]. Allergic rhinitis is a common disease of complex inheritance and is characterized by mucosal inflammation caused by allergen exposure.
  • Sequence similarities

    Contains 1 fork-head DNA-binding domain.
  • Developmental stage

    Expressed in developing lung, kidney and central nervous system.
  • Cellular localization

    Nucleus.
  • Target information above from: UniProt accession Q92949 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 2302 Human
    • Entrez Gene: 15223 Mouse
    • Entrez Gene: 116557 Rat
    • Omim: 602291 Human
    • SwissProt: Q92949 Human
    • SwissProt: Q61660 Mouse
    • SwissProt: Q63247 Rat
    • Unigene: 651204 Human
    • Unigene: 378938 Mouse
    • Unigene: 202954 Rat
    see all
  • Alternative names

    • FKHL13 antibody
    • Forkhead box J1 antibody
    • Forkhead box protein J1 antibody
    • Forkhead Homologue 4 antibody
    • Forkhead related protein FKHL13 antibody
    • Forkhead-related protein FKHL13 antibody
    • Fox J1 antibody
    • Foxj1 antibody
    • FOXJ1_HUMAN antibody
    • Hepatocyte nuclear factor 3 forkhead homolog 4 antibody
    • HFH 4 antibody
    • HFH-4 antibody
    • HFH4 antibody
    • HNF 3/forkhead homolog 4 antibody
    • MGC35202 antibody
    see all

Images

  • Western blot - Anti-FoxJ1 antibody (ab108452)
    Western blot - Anti-FoxJ1 antibody (ab108452)
    All lanes : Anti-FoxJ1 antibody (ab108452) at 1/250 dilution

    Lane 1 : A549 (Human lung adenocarcinoma epithelial cell line) Whole Cell Lysate
    Lane 2 : A549 (Human lung adenocarcinoma epithelial cell line) Whole Cell Lysate with Immunizing peptide at 1/250 dilution

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 45 kDa
    Observed band size: 49 kDa
    why is the actual band size different from the predicted?


    Exposure time: 8 minutes

Protocols

  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

    • Datasheet
    • SDS
  • References (0)

    Publishing research using ab108452? Please let us know so that we can cite the reference in this datasheet.

    ab108452 has not yet been referenced specifically in any publications.

    Images

    • Western blot - Anti-FoxJ1 antibody (ab108452)
      Western blot - Anti-FoxJ1 antibody (ab108452)
      All lanes : Anti-FoxJ1 antibody (ab108452) at 1/250 dilution

      Lane 1 : A549 (Human lung adenocarcinoma epithelial cell line) Whole Cell Lysate
      Lane 2 : A549 (Human lung adenocarcinoma epithelial cell line) Whole Cell Lysate with Immunizing peptide at 1/250 dilution

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 45 kDa
      Observed band size: 49 kDa
      why is the actual band size different from the predicted?


      Exposure time: 8 minutes

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    Alternative products to Anti-FoxJ1 antibody (ab108452)

    •  
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      Applications: IHC-P

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      Applications: IHC-P

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      Applications: IHC-P

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      Applications: ICC, IHC-P

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