Anti-FoxJ1 antibody [CL3989] (ab220028)
Key features and details
- Mouse monoclonal [CL3989] to FoxJ1
- Suitable for: IHC-P, ICC
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-FoxJ1 antibody [CL3989]
See all FoxJ1 primary antibodies -
Description
Mouse monoclonal [CL3989] to FoxJ1 -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species ICC HumanIHC-P Human -
Immunogen
Recombinant fragment corresponding to Human FoxJ1 aa 150-350.
Database link: Q92949 -
Epitope
Binds to an epitope located within the peptide sequence EPGKGGFWRI (aa 188-197) as determined by overlapping synthetic peptides. -
Positive control
- IHC-P: Human fallopian tube and endometrium tissues. ICC: BEWO cells
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General notes
This product was previously labelled as HFH4
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
CL3989 -
Isotype
IgG1 -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FoxJ1 antibody [CL3989] (ab220028)
Immunohistochemical analysis of paraffin-embedded human fallopian tube tissue labeling FoxJ1 with ab220028 at 1/1000 dilution.
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Immunocytochemistry/ Immunofluorescence analysis of BEWO cells labeling FoxJ1 with ab220028 at 2-10 µg/mL. Cells were fixed with PFA and permeabilised with Triton X-100. The data is showing specific staining in the nucleoplasm in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FoxJ1 antibody [CL3989] (ab220028)
Immunohistochemical analysis of paraffin-embedded human endometrium tissue labeling FoxJ1 with ab220028 at 1/1000 dilution.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FoxJ1 antibody [CL3989] (ab220028)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue labelling FoxJ1 with ab220028 at 1/1000 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-FoxJ1 antibody [CL3989] (ab220028)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human skeletal muscle tissue labelling FoxJ1 with ab220028 at 1/1000 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.