Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-PCSK2 antibody [EPR23578-19] - BSA and Azide free (ab274427)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23578-19] to PCSK2 - BSA and Azide free
  • Suitable for: IHC-P, Flow Cyt (Intra), ICC, WB
  • Reacts with: Human

Overview

  • Product name

    Anti-PCSK2 antibody [EPR23578-19] - BSA and Azide free
    See all PCSK2 primary antibodies

  • Description

    Rabbit monoclonal [EPR23578-19] to PCSK2 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, Flow Cyt (Intra), ICC, WBmore details
    Unsuitable for: IHC-Fr or IP

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: TT whole cell lysate; Human brain tissue lysate. IHC-P: Human pancreas, colon and adrenal gland tissue. ICC: TT cells. Flow Cyt (intra): TT cells.

  • General notes

    ab274427 is the carrier-free version of a274418.

    Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

    This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-PCSK2 antibody [EPR23578-19] - BSA and Azide free (ab274427)
    Western blot - Anti-PCSK2 antibody [EPR23578-19] - BSA and Azide free (ab274427)
    Anti-PCSK2 antibody [EPR23578-19] (ab274418) at 1/1000 dilution + Human brain tissue lysate at 40 µg

    Secondary
    VeriBlot for IP secondary antibody(HRP)(ab131366) at 1000 cells

    Predicted band size: 71 kDa
    Observed band size: 55 kDa
    why is the actual band size different from the predicted?



    This data was developed using ab274418, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure time: 3 minutes.

    55-kDa PC2 is observed. The molecular weight observed is consistent with what has been described in the literature (PMID: 8557169, 14693708).
    This blot was developed using a higher sensitivity ECL substrate.

  • Western blot - Anti-PCSK2 antibody [EPR23578-19] - BSA and Azide free (ab274427)
    Western blot - Anti-PCSK2 antibody [EPR23578-19] - BSA and Azide free (ab274427)
    Anti-PCSK2 antibody [EPR23578-19] (ab274418) at 1/1000 dilution + TT (human thyroid carcinoma epithelial cell) whole cell lysate at 40 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/50000 dilution

    Predicted band size: 71 kDa
    Observed band size: 70 kDa why is the actual band size different from the predicted?



    This data was developed using ab274418, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

    Exposure time: 37 seconds.

    70-kDa pro-PC2 is observed. The molecular weight observed is consistent with what has been described in the literature (PMID:8557169, 14693708).

  • Flow Cytometry - Anti-PCSK2 antibody [EPR23578-19] - BSA and Azide free (ab274427)
    Flow Cytometry - Anti-PCSK2 antibody [EPR23578-19] - BSA and Azide free (ab274427)

    This data was developed using ab274418, the same antibody clone in a different buffer formulation.

    Flow cytometric analysis of 4% paraformaldehyde fixed, 90% methanol permeabilized TT (Human thyroid carcinoma epithelial cell) cells labelling PCSK2 with ab274418 at 1/500 dilution (0.1ug) (Red) compared with a Rabbit monoclonal IgG (ab172730) isotype control (Black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

    A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

  • Immunocytochemistry - Anti-PCSK2 antibody [EPR23578-19] - BSA and Azide free (ab274427)
    Immunocytochemistry - Anti-PCSK2 antibody [EPR23578-19] - BSA and Azide free (ab274427)

    This data was developed using ab274418, the same antibody clone in a different buffer formulation.

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized TT cells labelling PCSK2 with ab274418 at 1/100 (5.25 ug/ml) dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing cytoplamic staining in TT cell line. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 dilution (Red). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCSK2 antibody [EPR23578-19] - BSA and Azide free (ab274427)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCSK2 antibody [EPR23578-19] - BSA and Azide free (ab274427)

    This data was developed using ab274418, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Human adrenal gland tissue labeling PCSK2 with ab274418 at 1/2000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Postive staining on human adrenal gland. The section was incubated with ab274418 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

     

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCSK2 antibody [EPR23578-19] - BSA and Azide free (ab274427)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCSK2 antibody [EPR23578-19] - BSA and Azide free (ab274427)

    This data was developed using ab274418, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling PCSK2 with ab274418 at 1/2000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Postive staining on myenteric neurons of human colon (PMID: 7829629). The section was incubated with ab274418 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCSK2 antibody [EPR23578-19] - BSA and Azide free (ab274427)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PCSK2 antibody [EPR23578-19] - BSA and Azide free (ab274427)

    This data was developed using ab274418, the same antibody clone in a different buffer formulation.

    Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labeling PCSK2 with ab274418 at 1/2000 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Postive staining on human pancreatic islets (PMID: 8916141, PMID: 7829629). The section was incubated with ab274418 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND. RX instrument Counterstained with Hematoxylin.

    Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

  • Anti-PCSK2 antibody [EPR23578-19] - BSA and Azide free (ab274427)
    Anti-PCSK2 antibody [EPR23578-19] - BSA and Azide free (ab274427)

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