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Rat Serpin A12 ELISA Kit (ab272197)

Key features and details

  • One-wash 90 minute protocol
  • Sensitivity: 3.79 pg/ml
  • Range: 23.44 pg/ml - 1500 pg/ml
  • Sample type: Cit plasma, EDTA Plasma, Serum
  • Detection method: Colorimetric
  • Assay type: Sandwich (quantitative)
  • Reacts with: Rat

Overview

  • Product name

    Rat Serpin A12 ELISA Kit
    See all Vaspin kits

  • Detection method

    Colorimetric

  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Serum 8 4.5%
    Inter-assay
    Sample n Mean SD CV%
    Serum 3 7.4%

  • Sample type

    Serum, EDTA Plasma, Cit plasma

  • Assay type

    Sandwich (quantitative)

  • Sensitivity

    3.79 pg/ml

  • Range

    23.44 pg/ml - 1500 pg/ml

  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Serum 86 83% - 90%
    EDTA Plasma 92 82% - 96%
    Cit plasma 89 85% - 94%

  • Assay time

    1h 30m

  • Assay duration

    One step assay

  • Species reactivity

    Reacts with: Rat

  • Product overview

    Rat Serpin A12 ELISA kit (ab272197) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Serpin A12 protein in serum and plasma. It uses our proprietary SimpleStep ELISA® technology. Quantitate Rat Serpin A12 with 3.79 pg/mL sensitivity.


    SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:


            -Single-wash protocol reduces assay time to 90 minutes or less


            -High sensitivity, specificity and reproducibility from superior antibodies


            -Fully validated in biological samples


            -96-wells plate breakable into 12 x 8 wells strips


    A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

  • Notes

    Serpins are a member of the serine protease inhibitor superfamily proteins that share similar structures. Serpin A12, also known as vaspin, was first identified in white adipose tissue of the OLETF (Otsuka Long-Evans Tokushima Fatty) rat Type 2 diabetes model. Moreover, as an adipokine, Serpin A12 modulates insulin action by specifically inhibiting its target protease KLK7. Studies have shown Serpin A12 to have multiple beneficial effects on metabolic, inflammatory, and atherogenic processes related to obesity. 

  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

Images

  • SimpleStep ELISA Protocol Diagram
    SimpleStep ELISA Protocol Diagram

    SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

  • Example of rat Serpin A12 standard curve in Sample Diluent NS.
    Example of rat Serpin A12 standard curve in Sample Diluent NS.

    The Serpin A12 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

  • Interpolated concentrations of native Serpin A12 in rat serum samples.
    Interpolated concentrations of native Serpin A12 in rat serum samples.

    The concentrations of Serpin A12 were measured in duplicates, interpolated from the Serpin A12 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Serpin A12 concentration was determined to be 489.1 pg/mL in serum.

  • Sandwich ELISA - Rat Serpin A12 ELISA Kit (ab272197)
    Sandwich ELISA - Rat Serpin A12 ELISA Kit (ab272197)
    To learn more about the advantages of recombinant antibodies see here.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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