Recombinant human IGF1 protein (Active) (ab270062)
Key features and details
- Expression system: HEK 293 cells
- Purity: >= 95% Immunogen affinity purified
- Endotoxin level:
- Active: Yes
- Suitable for: Cell Culture, SDS-PAGE, HPLC, MS, Functional Studies
Preparation and Storage
- IBP1
- IGF I
- IGF IA
Images
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Functional Studies - Recombinant human IGF1 protein (Active) (ab270062)
Fully biologicaly active as determined by dose-depended proliferation of MCF-7 cells. The ED50 is ≤ 0.9 ng/mL, corresponding to a specific activity of 1.11 x 106 units/mg.
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SDS-PAGE - Recombinant human IGF1 protein (Active) (ab270062)SDS-PAGE analysis of ab270062.
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HPLC - Recombinant human IGF1 protein (Active) (ab270062)Purity ≥95%
The spectrum was recorded using a 1260 Infinity II HPLC system with DAD and a MabPac RP column (3.0x100 mm, 4 µm). 5 µL of purified protein was injected and the gradient run from 80 % water:TFA (99.9:0.1 v/v) and 20 % acetonitrile:water:TFA (90:9.9:0.1 v/v/v) to 20 % water:TFA (99.9:0.1 v/v) and 80 % acetonitrile:water:TFA (90:9.9:0.1 v/v/v) within 3 minutes followed by an isocratic step for another 3 min. Flow rate was 0.5 mL/min and the column compartment temperature was 50 °C.
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Mass Spectrometry - Recombinant human IGF1 protein (Active) (ab270062)M - 2.84 Da (Calculated mass 7711.84 Da)
The spectrum was recorded with a 6545XT AdvanceBio LC/Q-TOF (Agilent Technologies) and a MabPac RP column (42.1x50 mm, 4 µm, Thermo Scientific). 5 µL of purified protein was injected and the gradient run from 85 % water:FA (99.9:0.1 v/v) and 15 % acetonitrile:FA (90:9.9:0.1 v/v/v) to 55 % water:FA (99.9:0.1 v/v) and 45 % acetonitrile:FA (90:9.9:0.1 v/v/v) within 3 minutes followed by an isocratic step for another 2.5 min. Flow rate was 0.4 mL/min and the column compartment temperature was 60 °C. Data was analysed and deconvoluted using the Bioconfirm software (Agilent Technologies).